Plant Vacuoles pp 425-433 | Cite as

Subcellular Localization of Primary and Second Cardiac Glycosides, and the Related 16′-O-Glucosyl-Transferase in Cell Suspension Cultures of Digitalis Lanata

  • Wolfgang Kreis
  • Ursula May
  • Ernst Reinhard
Part of the NATO ASI Series book series (NSSA, volume 134)


Plant cell cultures, like microorganisms, are able to effect structural modifications on exogenously supplied steroids (Reinhard, 1974; Stochs, 1980). In this context the biotransformation of cardiac glycosides by suspension-cultured Digitalis lanata cells (Reinhard et al., 1975) is of special interest because of the world-wide use of these compounds in the treatment of heart diseases.


Plant Cell Culture Cardiac Glycoside Nylon Cloth Vacuolar Storage Cell Culture Process 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alfermann, A. W., Bergmann, W., Figur, C., HelmUnderline, U., Schwantag, D., Schuller, I., and Reinhard, E., 1983, Biotransformation of β-methyldigitoxin to β-methyldigoxin by cell cultures of Digitalis lanata, in: “Plant Biotechnology”, S. H. Mantell and H. Smith, eds., Cambridge University Press, Cambridge.Google Scholar
  2. Alibert, G., Boudet, A. M., and Rataboul, P., 1982, Transport of o-coumaric acid glucoside in isolated vacuoles of sweet cover, in: “Plasmalemma and Tonoplast: Their Functions in the Plant Cell”, D. Marmé, E. Marré, and R. Hertel, eds., Elsevier, Amsterdam.Google Scholar
  3. Bergmeyer, H. U., 1974, “Methoden der Enzymatischen Analyse”, 3. Aufl., Verlag Chemie, Weinheim.Google Scholar
  4. Boller, T., 1982, Enzymatic equipment of plant vacuoles, Physiol. Vég., 20: 247.Google Scholar
  5. Bradford, M. M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72: 248.Google Scholar
  6. Deus-Neumann, B., and Zenk, M., 1984, A highly selective alkaloid uptake system in vacuoles of higher plants, Planta, 162: 250.CrossRefGoogle Scholar
  7. Heins, M., 1978, Screening of Digitalis lanata plants and cell cultures for hydroxylation capacity, in: “Production of Natural Compounds by Cell Culture Methods”, A. W. Alfermann and E. Reinhard, eds., Gesellschaft für Strahlen und Umweltforschung mbH, München.Google Scholar
  8. Kreis, W., and Reinhard, E., 1985, Rapid isolation of vacuoles from suspension of cultured Digitalis lanata cells, J. Plant Physiol., 121: 385.Google Scholar
  9. Kreis, W., May, U., and Reinhard, E., 1986, UDP-glucose:digitoxin 16’-O-glucosyltransferase from suspension-cultured Digitalis lanata cells, Plant Cell Reports, submitted.Google Scholar
  10. Löffelhardt, W., and Kopp, B., 1981, Subcellular localization of glucosyltransferases involved in cardiac glycoside glucosylation in leaves of Convallaria majalis, Phytochemistry, 20: 1219.CrossRefGoogle Scholar
  11. Matile, P., 1984, Das toxische Kompartment der Pflanzenzelle, Naturwiss., 71: 18.Google Scholar
  12. Murashige, T., and Skoog, F., 1962, A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 15: 473.Google Scholar
  13. Ohlrogge, J. B., Garcia-Martinez, J. L., Adams, D., and Rappaport, L., 1980, Uptake and subcellular compartmentation of gibberellin Al applied to leaves of barley and cowpea, Plant Physiol., 66: 422.Google Scholar
  14. Petersen, M., and Seitz, H. U., 1985, Cytochrome P-450-dependent digitoxin 12-β-hydroxylase from cell cultures of Digitalis lanata, FEBS Letters, 188:11.Google Scholar
  15. Reinhard, E., 1974, Biotransformations by plant tissue cultures, in: “Tissue culture and Plant Science 1974”, H. E. Street, ed., Academic Press, London.Google Scholar
  16. Reinhard, E., Boy, H. M., and Kaiser, F., 1975, Umwandlungen von Digitalis Glykosiden durch Zellsuspensionskulturen, Planta Med. Suppl., 163.Google Scholar
  17. Stohs, S. J., 1980, Metabolism of steroids in plant tissue cultures, in: “Advances in Biochemical Engineering”, Vol. 16, Plant cell cultures I, A. Fiechter, ed., Springer-Verlag, Berlin.Google Scholar
  18. Schmitt, R., and Sandermann, H., 1982, Specific localization of 13-D-glucoside conjugates of 2,4-dichlorophenoxyacetic acid in soybean vacuoles, Z. Naturforsch., 37c: 772.Google Scholar
  19. Wagner, G., 1983, Higher plant vacuoles and tonoplasts, in: “Isolation of Membranes and Organelles from Plant Cells”, J. L. Hall and A. L. Moore, eds., Academic Press, London.Google Scholar
  20. Werner, C., and Matile, P., 1984, Accumulation of coumarylglucosides in vacuoles of barley mesophyll protoplasts, J. Plant Physiol., 118: 237.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Wolfgang Kreis
    • 1
  • Ursula May
    • 1
  • Ernst Reinhard
    • 1
  1. 1.Pharmazeutisches InstitutUniversität TübingenTübingenGermany

Personalised recommendations