Abstract
Plant cell cultures, like microorganisms, are able to effect structural modifications on exogenously supplied steroids (Reinhard, 1974; Stochs, 1980). In this context the biotransformation of cardiac glycosides by suspension-cultured Digitalis lanata cells (Reinhard et al., 1975) is of special interest because of the world-wide use of these compounds in the treatment of heart diseases.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Alfermann, A. W., Bergmann, W., Figur, C., HelmUnderline, U., Schwantag, D., Schuller, I., and Reinhard, E., 1983, Biotransformation of β-methyldigitoxin to β-methyldigoxin by cell cultures of Digitalis lanata, in: “Plant Biotechnology”, S. H. Mantell and H. Smith, eds., Cambridge University Press, Cambridge.
Alibert, G., Boudet, A. M., and Rataboul, P., 1982, Transport of o-coumaric acid glucoside in isolated vacuoles of sweet cover, in: “Plasmalemma and Tonoplast: Their Functions in the Plant Cell”, D. Marmé, E. Marré, and R. Hertel, eds., Elsevier, Amsterdam.
Bergmeyer, H. U., 1974, “Methoden der Enzymatischen Analyse”, 3. Aufl., Verlag Chemie, Weinheim.
Boller, T., 1982, Enzymatic equipment of plant vacuoles, Physiol. Vég., 20: 247.
Bradford, M. M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72: 248.
Deus-Neumann, B., and Zenk, M., 1984, A highly selective alkaloid uptake system in vacuoles of higher plants, Planta, 162: 250.
Heins, M., 1978, Screening of Digitalis lanata plants and cell cultures for hydroxylation capacity, in: “Production of Natural Compounds by Cell Culture Methods”, A. W. Alfermann and E. Reinhard, eds., Gesellschaft für Strahlen und Umweltforschung mbH, München.
Kreis, W., and Reinhard, E., 1985, Rapid isolation of vacuoles from suspension of cultured Digitalis lanata cells, J. Plant Physiol., 121: 385.
Kreis, W., May, U., and Reinhard, E., 1986, UDP-glucose:digitoxin 16’-O-glucosyltransferase from suspension-cultured Digitalis lanata cells, Plant Cell Reports, submitted.
Löffelhardt, W., and Kopp, B., 1981, Subcellular localization of glucosyltransferases involved in cardiac glycoside glucosylation in leaves of Convallaria majalis, Phytochemistry, 20: 1219.
Matile, P., 1984, Das toxische Kompartment der Pflanzenzelle, Naturwiss., 71: 18.
Murashige, T., and Skoog, F., 1962, A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 15: 473.
Ohlrogge, J. B., Garcia-Martinez, J. L., Adams, D., and Rappaport, L., 1980, Uptake and subcellular compartmentation of gibberellin Al applied to leaves of barley and cowpea, Plant Physiol., 66: 422.
Petersen, M., and Seitz, H. U., 1985, Cytochrome P-450-dependent digitoxin 12-β-hydroxylase from cell cultures of Digitalis lanata, FEBS Letters, 188:11.
Reinhard, E., 1974, Biotransformations by plant tissue cultures, in: “Tissue culture and Plant Science 1974”, H. E. Street, ed., Academic Press, London.
Reinhard, E., Boy, H. M., and Kaiser, F., 1975, Umwandlungen von Digitalis Glykosiden durch Zellsuspensionskulturen, Planta Med. Suppl., 163.
Stohs, S. J., 1980, Metabolism of steroids in plant tissue cultures, in: “Advances in Biochemical Engineering”, Vol. 16, Plant cell cultures I, A. Fiechter, ed., Springer-Verlag, Berlin.
Schmitt, R., and Sandermann, H., 1982, Specific localization of 13-D-glucoside conjugates of 2,4-dichlorophenoxyacetic acid in soybean vacuoles, Z. Naturforsch., 37c: 772.
Wagner, G., 1983, Higher plant vacuoles and tonoplasts, in: “Isolation of Membranes and Organelles from Plant Cells”, J. L. Hall and A. L. Moore, eds., Academic Press, London.
Werner, C., and Matile, P., 1984, Accumulation of coumarylglucosides in vacuoles of barley mesophyll protoplasts, J. Plant Physiol., 118: 237.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Plenum Press, New York
About this chapter
Cite this chapter
Kreis, W., May, U., Reinhard, E. (1987). Subcellular Localization of Primary and Second Cardiac Glycosides, and the Related 16′-O-Glucosyl-Transferase in Cell Suspension Cultures of Digitalis Lanata . In: Marin, B. (eds) Plant Vacuoles. NATO ASI Series, vol 134. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5341-6_55
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5341-6_55
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5343-0
Online ISBN: 978-1-4684-5341-6
eBook Packages: Springer Book Archive