Vacuolar and Cytosolic Metabolite Pools by Comparative Fractionation of Vacuolate and Evacuolate Protoplasts
Knowledge about the distribution of metabolites between subcellular compartments is important for the understanding of regulatory events. Orthophosphate (Pi), e.g., is involved in most biochemical processes of energy transduction within the cell, thereby affecting important metabolic routes such as respiration, glycolysis or photosynthesis. Especially partioning of photosynthetically fixed CO2 between starch and sucrose appears to be regulated by the stromal and cytosolic pool sizes of Pi.
KeywordsMesophyll Protoplast Pyridine Nucleotide Glycolate Oxidase Vacuolar Compartment Phosphate Status
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- Hampp, R., 1985, ADP and AMP, Luminometric method, in: “Methods in Enzymatic Analysis”, Vol. 7, J. Bergmeyer and M. Grassl, eds., Verlag Chemie, Weinheim.Google Scholar
- Hampp, R., Goller, M., Füllgraf, H., and Eberle, I., 1985, Pyridine and adenine nucleotide status, and pool sizes of a range of metabolites in chloroplasts, mitochondria and the cytosol/vacuole of Avena mesophyll protoplasts during dark/light transition: Effect of pyridoxal phosphate, Plant Cell Physiol., 26: 99.Google Scholar
- Hampp, R., Goller, M., and Ziegler, H., 1982, Adenylate levels, energy charge and phosphorylation potential during dark/light and light/dark transition in chloroplasts, mitochondria and cytosol of mesophyll protoplasts from Avena sativa L., Planta, 62: 448.Google Scholar
- Kato, T., Berger, S. J., Carter, J. A., and Lowry, O. H., 1973, An enzymatic cycling method for nicotine adenine dinucleotide with malate and alcohol dehydrogenase, Anal. Biochem., 53: 86.Google Scholar
- Lowry, O. H., and Passonneau, J. V., 1972, “A flexible system of enzymatic analysis”, Academic Press, New-York.Google Scholar