Plant Vacuoles pp 393-400 | Cite as

The Possible Role of Endoplasmic Reticulum in the Biosynthesis and Transport of Anthocyanin Pigments

  • George J. Wagner
Part of the NATO ASI Series book series (NSSA, volume 134)


The availability of methods for isolating higher plant vacuoles from a variety of plant tissues has led to recent studies of the compartmentation and mechanisms of accumulation of various plant secondary metabolites. Long suspected and inferred vacuolar location of many secondary metabolites has been confirmed in studies of vacuoles/extravacuole compartmentation using protoplasts and vacuoles isolation after slicing of root tissues (Matile, 1984; Ryan and Walker-Simmons; Wagner, 1985). More recently, a number of investigators have asked questions about which secondary products are taken up by isolated vacuoles prepared from which species, and what chemical and stereochemical-conformational properties are required for metabolite uptake into isolated vacuoles in vitro ((Deus-Neumann and Zenk, 1986; Matern et al., 1986; Werner and Matile, 1985; Rataboul et al., 1985). The results of these studies are quite interesting, but at present, it is not clear if these experiments elucidate the in vivo mechanism(s) for vacuolar accumulation of secondary metabolites. Much additional work is needed and is not doubt forthcoming.


Secondary Metabolite Rough Endoplasmic Reticulum Secondary Product Anthocyanin Pigment Isoquinoline Alkaloid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Amann, M., Wanner, G., and Zenk, M. H., 1986, Intracellular compartmentation of two enzymes of berberine biosynthesis in plant cell cultures, Planta, 167: 310.CrossRefGoogle Scholar
  2. Conn, E. E., 1984, Compartmentation of secondary compounds, in: “Proceedings of the Phytochemical Society of Europe”, A. M. Boudet, G. Alibert, G.Google Scholar
  3. Marigo, and P. J. Rea, eds., Vol. 24, 1, Clarendon Press, Oxford. Deus-Neumann, B., and Zenk, M. H., 1986, Accumulation of alkaloids in plant vacuo-les does not involve an ion-trap mechanism, Planta, 167:44.Google Scholar
  4. Guilliermond, A., 1941, “The Cytoplasm of the Plant Cell”, Chronica Botanica, Waltham, Massachussetts.Google Scholar
  5. Hrazdina, G., and Wagner, G. J., 1985, Compartmentation of plant phenolic compounds: Sites of synthesis and accumulation, in: “Proceedings of the Phytochemical Society of Europe”, C. F. Sumere, and P. J. Lea, eds., Vol. 25, p. 119,Clarendon Press, Oxford.Google Scholar
  6. Matern, V., Reichenbach, C., and Heller, W., 1986, Efficient uptake of flavanoids into parsley vacuoles requires acetylated glycosides, Planta, 167: 183.CrossRefGoogle Scholar
  7. Masters, C., 1984, Interactions between glycolytic enzymes and components of cytomatrix, J. Cell. Biol., 99: 222.Google Scholar
  8. Matile, P., 1984, Das toxische kompartiment der pflanzell, Naturwissen., 71: 18.CrossRefGoogle Scholar
  9. Nessler, C. L., and Mahlberg, P. G., 1977, Ontogeny and cytochemistry of alkaloidal vesicles in laticifers of Papaver somniferum L., Amer. J. Bot., 64: 541.Google Scholar
  10. Paine, P. L., 1984, Diffusive and non-diffusive proteins, in vivo, JJ. Cell. Biol., 99: 219.Google Scholar
  11. Parham, R. A., and H. M. Kaustinen, H. M., 1977, On the site of tannin synthesis in plant cells, Bot. Gaz., 138: 465.Google Scholar
  12. Rataboul, P., Alibert, G., Boller, T., and Boudet, A. M., 1985, Intracellular transport and vacuolar accumulation of o-coumaric acid glucoside in Melilotus alba mesophyll cell protoplasts, Biochim. Biophys. Acta, 816: 25.Google Scholar
  13. Renaudin, J. P., and Guern, J., 1982, Compartmentation mechanisms of indole alkaloids in cell suspension cultures of Catharanthus roseus, Physiol. Vég., 20: 533.Google Scholar
  14. Russell, D. W., 3-hydroxy-3-methylglutaryl-CoA reductases from pea seedlings, Methods in Enzymol., 110: 26.Google Scholar
  15. Ryan, C. A., and Walker-Simmons, M., 1983, Methods in Enzymol., 96: 580.CrossRefGoogle Scholar
  16. Stafford, H. A., 1981, Compartmentation in natural product biosynthesis by multi-enzyme complexes, in: “The Biochemistry of Plants”, E. E. Conn, ed.,Vol. 7, p. 117, Academic Press, New-York.Google Scholar
  17. Tsukada, M., and Tabata, M., 1984, Intracellular localization and secretion of naphtoquinone pigments in cell cultures of Lithospermum erythrorhizon, Planta Medica, 51: 338.CrossRefGoogle Scholar
  18. Van Sumere, C. F., Albrecht, J., Dedonder, A., DePooter H., and I. Pe’, 1975, in: “The Chemistry and Biochemistry of Plant Proteins”, J. Harborne, and C. F., Van Sumere, eds., p. 211, Academic Press, New-York and London.Google Scholar
  19. Wagner, G. J., 1981, Compartmentation in plant cells: The role of the vacuole, in: “Recent Advances in Phytochemistry”, L. L. Creasy, and G. Hrazdina, eds., Vol. 16, p. 1, Plenum Press, New-York and London.Google Scholar
  20. Wagner, G., 1985, in: “Modern Methods of Plant Analysis”, H. F. Liskins, and J. F. Jackson, eds., Vol. 1, p. 105, Springer-Verlag, Berlin, Heidelberg, New-York, and Tokyo.Google Scholar
  21. Wagner, G. J., and Hrazdina, G., 1984, Endoplasmic reticulum as a site of phenyl-propanoid and flavanoid metabolism in Hippeastrum, Plant Physiol., 74: 901.PubMedCrossRefGoogle Scholar
  22. Werner, C., and Matile, P., 1985, Accumulation of coumaryl-glucosides in vacuoles of barley mesophyll protoplasts, J. Plant. Physiol., 118: 237.Google Scholar
  23. West, C. A., Dudley, M. W., and Dueber, M. T., 1979, Regulation of terpenoid biosyn-thesis in higher plants, in: “Recent Advances in Phytochemistry”, T. Swain and G. R. Waller, eds., Vol. 13, p. 163, Plenum Press, New-York and London. Wombacher, H., 1983, Molecular compartmentation by enzyme cluster formation,Mol. Cell Biochem., 56: 155.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • George J. Wagner
    • 1
  1. 1.Agronomy DepartmentUniversity of LexingtonLexingtonUSA

Personalised recommendations