Plant Vacuoles pp 383-391 | Cite as

Compartmentation of Glycosidases in a Light Vacuole Fraction from the Latex of Lactuca Sativa L.

  • Roger Giordani
  • Georges Noat
  • Francis Marty
Part of the NATO ASI Series book series (NSSA, volume 134)


Articulated laticifers in young growing roots of Lactuca sativa L. arise from aligned sets of cells, the end walls as well as local areas of transverse walls of which disintegrate when branched tubes are formed with adjacent cells (Giordani, 1977 and 1979). Before being completely perforated cell walls become locally thinner. Simultaneously, numerous membrane vesicles originating from the plasmalemma and loaded with polysaccharides are present in the neighboring cytoplasm and some have been observed inside the vacuoles (Giordani, 1980). Because of the general lytic function of vacuoles (Marty et al., 1980; Matile, 1978) the results of our previous experiments suggest that cell wall components carried by endocytic vesicles might be digested in the vacuoles of the latex in differentiating laticifers of Lactuca sativa. Electron microscope cytochemistry has previously shown a marked increase in cellulase and acid hydrolase activities in cell wall areas while these are being remodeled (Giordani, 1980 and 1981). Cellulase and acid phosphatase activities have also been detected in small membrane vesicles (Giordani, 1980).


Cellulase Activity Papaver Somniferum Cell Wall Fragment Pectinase Activity Glycosidase Activity 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Roger Giordani
    • 1
  • Georges Noat
    • 1
  • Francis Marty
    • 2
  1. 1.Centre de Biochimie et de Biologie MoléculaireC.N.R.S.Marseille Cedex 9France
  2. 2.Laboratoire de Biologie de la Différenciation CellulaireFaculté des Sciences de LuminyMarseille-Cedex 9France

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