Plant Vacuoles pp 369-380 | Cite as

Protein Degradation in Vacuoles from Acer Pseudoplatanus L. Cells

  • Hervé Canut
  • Gilbert Alibert
  • Antoine Carrasco
  • Alain M. Boudet
Part of the NATO ASI Series book series (NSSA, volume 134)


In higher plants, intracellular protein breakdown is a fundamental process which has important implications in biochemical regulation, protein turnover (Davies, 1982) and physiological events such as germination (Mikola, 1983) or senescence (Thimann, 1980; Laurière, 1983). However, the mechanisms that control the degradation of proteins are still obscure even though the characterization of plant proteolytic enzymes, and particularly their intracellular location has been extensively studied.


Acer Cell Abnormal Protein Vacuolar Protein Vacuolar Compartment Intracellular Protein Degradation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alibert, G., Carrasco, A., and Boudet, A. M., 1982, Changes in biochemical composition of vacuoles isolated from Acer pseudoplatanus L. during cell culture, Biochim. Biophys. Acta, 721:22.CrossRefGoogle Scholar
  2. Ashwell, G., and Morell, A. G., 1974, The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins, Adv. Enzymol., 41:99.PubMedGoogle Scholar
  3. Boller, T., 1982, Enzymatic equipment of plant vacuoles, Physiol. Vég., 20:247.Google Scholar
  4. Canut, li., Alibert, G., and Boudet, A. M., 1985, Proteases of Melilotus alba mesophyll protoplasts: I. Intracellular localization. Plant Science Letters, 39:163.CrossRefGoogle Scholar
  5. Canut, H., Alibert, G., and Boudet, A. M., 1985 b, Hydrolysis of intracellular proteins in vacuoles isolated from Acer pseudoplatanus L. cells, Plant Physiol., 79:1090.CrossRefGoogle Scholar
  6. Canut, H., Alibert, G., Carrasco, A., and Boudet, A. M., 1986, Rapid degradation of abnormal proteins in vacuoles from Acer pseudoplatanus L. cells, Plant Physiol, 81, in press.Google Scholar
  7. Canut, H., Alibert, G. and Carrasco, A., 1986, Intracellular degradation of glycoproteins in Acer pseudoplatanus L. cells, submitted.Google Scholar
  8. Coates, J. B., and Davies, D. D., 1983, The molecular basis of the selectivity of protein degradation in stressed senescent barley (Hordeum vulgare cv. Proctor) leaves, Planta, 158:550.CrossRefGoogle Scholar
  9. Davies, D. D., 1982, Physiological aspects of protein turnover, in: “Encyclopedia of Plant Physiology, New Series, Vol. 14 A: Nucleic Acids and Proteins in Plants”, D. Boulder and B. Parthier, eds., Springer-Verlag, Berlin.Google Scholar
  10. Etlinger, J. D., and Goldberg, A. L., 1977, A soluble ATP-dependent proteolytic system responsible for the degradation of abnormal proteins in reticulocytes, Proc. Natl. Acad. Sci. USA, 74:54.PubMedCrossRefGoogle Scholar
  11. Goldberg, A. L., and St-John, A. C., 1976, Intracellular protein degradation in mammalian and bacterial cells: Part 2, Annu. Rev. Bochem., 45:747.CrossRefGoogle Scholar
  12. Hershko, A., Eytan, E., Ciechanover, A., and Haas, A. L., 1982, Immunochemical analysis of the turnover of ubiquitin protein conjugates in intact cells. Relationship to the breakdown of abnormal proteins, J. Biol. Chem., 257:13964.PubMedGoogle Scholar
  13. Kurkdjian, A., 1982, Absorption and accumulation of nicotine by Acer pseudoplatanus and Nicotiana tabacum cells, Physiol. Vég., 20:73.Google Scholar
  14. Kurkdjian, A., and Guern, J., 1981, Vacuolar pH measurement in higher plant cells. I. Evaluation of the methylamine method, Plant Physiol., 67:953.PubMedCrossRefGoogle Scholar
  15. Laurière, C., 1983, Enzymes and leaf senescence, Physiol. Vég., 21:1159.Google Scholar
  16. Matile, P., 1975, The lytic compartment of plant cells, M. Alfert, W. Beerman, G. Rudkin, W. Saudritter and P. Stittle, eds., Vol. 1, Springer-Verlag, Wien, New-York.Google Scholar
  17. Matile, P., 1978, Biochemistry and function of vacuoles, Annu. Rev. Plant Physiol., 29:193.CrossRefGoogle Scholar
  18. Matile, P. and Wikenbach, F., 1971, Function of lysosomes and lysosomal enzymes in the senescing corolla of the morning glory (Ipomea purpurea), J. Expt. Bot., 22:759.CrossRefGoogle Scholar
  19. Mikola, J., 1983, Proteinases, peptidases, and inhibitors of endogenous proteinases in germinating seeds, in: “Seed proteins”, J. Daussant, J. Mossé, and J. Vaughan, eds., Ann. Proc. The Phytochemical Society of Europe, Vol. 20, Academic Press, London.Google Scholar
  20. Nishimura, M., and Beevers, H., 1979, Hydrolysis of protein in vacuoles isolated from higher plant tissue, Nature, 277:412.CrossRefGoogle Scholar
  21. Olden, K., Parent, J. B., and White, S. L., 1982, Carbohydrate moieties of glycoproteins. A reevaluation of their function, Biochim. Biophys. Acta, 650:209.PubMedGoogle Scholar
  22. Olden, K., Bernard, B. A., Humphries, M. J., Yeo, T. K., White, S. L., Newton, S. A., Bauer, H. C., and Parent, J. B., 1985, Function of glycoprotein glycans, Trends Biochem. Sci., 10:78.CrossRefGoogle Scholar
  23. Rataboul, P., Alibert, G., Boller, T., and Boudet, A. M., 1985, Intracellular transport and vacuolar accumulation of o-coumaric acid glucoside in Melilotus alba mesophyll cell protoplasts, Biochim. Biophys. Acta, 816:25.CrossRefGoogle Scholar
  24. Thimann, K. V., 1980, The senescence of leaves, in: “Senescence in Plants”, R. C. Aldeman and G. S. Roth, eds., CRC Series in Ageing, CRC Press, Boca Raton, Florida.Google Scholar
  25. Van der Wilden, W., Herman, E. M. and Chrispeels, M. J., 1980, Protein bodies of mung bean cotyledons as autophagic organelles. Proc. Natl. Acad. Sci. USA, 77:428.PubMedCrossRefGoogle Scholar
  26. Wharton, S. A., and Hipkiss, A. R., 1984, Abnormal proteins of shortened length are preferentially degraded in the cytosol of cultured MRC5 fibroblasts, FEBS Letters, 168:134.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Hervé Canut
    • 1
  • Gilbert Alibert
    • 1
  • Antoine Carrasco
    • 1
  • Alain M. Boudet
    • 1
  1. 1.Centre de Physiologie VégétaleUniversité Paul SabatierToulouse-CedexFrance

Personalised recommendations