Role of the Vacuole in Metal Ion Trapping as Studied by in Vivo31P-NMR Spectroscopy
31P-NMR spectroscopy has been extensively used to study the energetic profiles in intact plant tissues (Roberts and Jardetzsky, 1981; Roberts, 1984; Loughman and Ratcliffe, 1984). These reports clearly demonstrate that this technique can be used to measure energy status, ATP/ADP ratio (Roberts et al., 1985), changes in intracellular pH (Roberts et al., 1981), effects of hypoxia (Roberts et al., 1984), phosphate movement (Rebeille et al., 1983), and aluminium ion toxicity (Pfeffer et al., 1986).
KeywordsTonoplast Membrane Intact Plant Tissue Energetic Profile Inversion Recovery Method Diphosphoric Acid
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