Plant Vacuoles pp 339-347 | Cite as

Ajmalicine Transport into Vacuoles Isolated from Catharanthus Roseus Cells

  • Jean-Pierre Renaudin
  • Jean Guern
Part of the NATO ASI Series book series (NSSA, volume 134)


We have studied previously the compartmentation of ajmalicine in Catharanthus roseus cells (Renaudin and Guern, 1982; Renaudin et al., 1985) and of nicotine in Nicotiana tabacum and Acer pseudoplatanus cells (Kurkdjian, 1982). The behavior of the 14C-labelled alkaloids added in the cell suspensions was in good agreement with the predictions of the so-called ion-trapping model: These alkaloids apparently diffused passively through the plasmalemma and the tonoplast, mainly under their neutral form, and did accordingly distribute between the cells and the medium. They were accumulated within cells, likely in vacuoles, against a concentration gradient, because of the relative acidity of the vacuolar compartment and of the low capacity for diffusion of the alkaloid cation. The distribution of alkaloids between the cells (i.e. the vacuoles) and the medium was dynamic and it was a function of 1) the acidity constant of the alkaloid, and 2) the pH difference between the vacuole and the medium. Due to the high pH (ca. 7.5) and low relative volume (less than 20%) of the cytoplasm, the accumulation of this compartment was likely neglectable. It was assumed that accumulation in whole cells merely reflected the accumulation within vacuoles, thus allowing the indirect approach of vacuolar properties.


Indole Alkaloid Tritiated Water Acidity Constant Vacuolar Compartment Plant Vacuole 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Barbier-Brygoo, H., Renaudin, J.-P., Manigault, P., Mathieu, Y., Kurkdjian, A., and Guern, J., 1987, Properties of vacuoles as a function of the isolation procedure, in: “Plant Vacuoles. Their Importance in Solute Compartmentation and Their Applications in Biotechnology”, B. Marin, ed., Plenum Publishing Corporation, New-York.Google Scholar
  2. Brown, S. C., Renaudin, J. P., Prévot, C., and Guern, J., 1984, Flow cytometry and sorting of plant protoplasts: Technical problems and physiological results from a study of pH and alkaloids in Catharanthus cells, Physiol. Vég., 22: 541.Google Scholar
  3. Deus-Neumann, B., and Zenk, M. H., 1984, A highly selective alkaloid uptake system in vacuoles of higher plants, Planta, 162: 250.CrossRefGoogle Scholar
  4. Deus-Neumann, B., and Zenk, M. H., 1986, Accumulation of alkaloids in plant vacuoles does not involve an ion-trap mechanism, Planta, 167: 44.CrossRefGoogle Scholar
  5. Guern, J., Renaudin, J. P., and Barbier-Brygoo, H., 1987, Accumulation of solutes in plant vacuoles: Interpretation of data is not so easy, in: “Plant Vacuoles. Their importance in Solute Compartmentation and their Applications in Biotechnology”, B. Marin, ed., Plenum Publishing Corporation, New-York.Google Scholar
  6. Heldt, H. W., 1980, Measurement of metabolite movement across the envelope and of the pH in the stroma and the thylakoid space in intact chloroplasts, Methods in Enzymology, 69: 604.CrossRefGoogle Scholar
  7. Kurkdjian, A. C., 1982, Absorption and accumulation of nicotine by Acer pseudoplatanus and Nicotiana tabaccum cells, Physiol. Vég., 20: 73.Google Scholar
  8. Kurkdjian, A. C., Quiquampoix, H., Barbier-Brygoo, H., Péan, M., Manigault, P., and Guern, J., 1985, Critical evaluation of methods for estimating the vacuolar pH of plant cells, in: “Biochemistry and Function of Vacuolar Adenosine-triphosphatase in Fungi and Plants”, B. P. Marin, ed., Springer-Verlag, Berlin, Heidelberg, New-York and Tokyo.Google Scholar
  9. Manigault, P., Manigault, J., and Kurkdjian, A. C., 1983, A microfluorimetric method for vacuolar pH measurement in plant cells using 9-aminoacridine, Physiol. Vég., 21: 129.Google Scholar
  10. Martinoia, E., Flügge, I., Kaiser, G., Heber, U., and Heldt, H. W., 1985, Energy-dependent uptake of malate into vacuoles isolated from barlkey mesophyll protoplasts, Biochim. Biophys. Acta, 806: 311.CrossRefGoogle Scholar
  11. Renaudin, J. P., 1981, Uptake and accumulation of an indole alkaloid, 14C-tabernanthine, by cell suspension cultures of Catharanthus roseus (L.) G. Don and Acer pseudoplatanus L., Plant Science Letters, 22: 59.CrossRefGoogle Scholar
  12. Renaudin, J. P., and Guern, J., 1982, Compartmentation mechanisms of indole alkaloids in cell suspension cultures of Catharanthus roseus, Physiol. Vég., 20: 533.Google Scholar
  13. Renaudin, J.-P., Brown, S. C., and Guern, J., 1985, Compartmentation of alkaloids in a cell suspension of Catharanthus roseus: A reappraisal of the role of pH gradients, in: “Primary and Secondary Metabolism of Plant Cell Cultures”, K. H. Neumann, W. Barz, and E. Reinhard, eds., Springer-Verlag, Berlin.Google Scholar
  14. Renaudin, J. P., Brown, S. C., Barbier-Brygoo, H., and Guern, J., 1986, Quantitative characterization of protoplasts and vacuoles from suspension cultured cells of Catharanthus roseus, Physiol. Plant.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Jean-Pierre Renaudin
    • 2
  • Jean Guern
    • 1
  1. 1.Laboratoire de Physiologie Cellulaire VégétaleCNRS/INRAGif-sur-YvetteFrance
  2. 2.Station de Physiopathologie VégétaleINRADijonFrance

Personalised recommendations