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Plant Vacuoles pp 297-300 | Cite as

Preliminary Evidence for Solubilization of an Active Tonoplast-Bound Vectorial Sucrose Synthesizing Enzyme Complex

  • Margaret Thom
  • Andrew Maretzki
Part of the NATO ASI Series book series (NSSA, volume 134)

Abstract

The existence of a UDP-glucose-dependent vectorial mechanism for the formation of vacuolar sucrose on the tonoplast has been demonstrated on a number of different plant species (Thom and Maretzki, 1985; Thom et al., 1986; Oleski et al., 1986). The evidence, so far, indicates that hexose phosphates cannot penetrate the tonoplast membrane any better than can free hexoses, sucrose, or sucrose-P (Thom and Maretzki, 1985; Maretzki and Thom, 1986). This implies that the appearance of labeled sucrose-P and free sucrose inside vacuoles from the external addition of UDPG-glucose labeled in the glucose moiety requires the formation of fructose-6 P from the added UDP-glucose. Indeed, this hypothesis is further strengthened by the fact that neither cold UDP-glucose added simultaneously with labeled fructose-6 P, nor UDP-glucose, labeled in the nucleotide moiety lead to the appearance of label associated with the vacuole (Thom and Maretzki, 1986).

Keywords

Pellet Fraction Detergent Concentration Protoplast Suspension Hexose Phosphate Tonoplast Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Maretzki, A., and Thom, M., 1986, A group translocator for sucrose assimilation in tonoplast vesicles of sugarcane cells, Plant Physiol. 80: 34.PubMedCrossRefGoogle Scholar
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  3. Thom, M., and Maretzki, A., 1985, Group translocation as a mechanism for sucrose transfer into vacuoles from sugarcane cells, Proc. Natl. Acad. Sci. U.S.A., 82: 4697.PubMedCrossRefGoogle Scholar
  4. Thom, M., and Maretzki, A., 1985, Group translocation as a mechanism for sucrose transfer into vacuoles from sugarcane cells, Proc. Natl. Acad. Sci. U.S.A., 82: 4697.PubMedCrossRefGoogle Scholar
  5. Thom, M., and Maretzki, A., 1986, Comparison of some characteristics of uridinediphosphate-glucose-dependent sucrose synthesis in isolated vacuoles and tonoplast vesicles, in: “Phloem Transport”, J. Cronshaw, W. J. Lucas, and R. T. Giaquinta, eds., A. R. Liss Inc., New-York.Google Scholar
  6. Thom, M., Leigh, L. A., and Maretzki, A., 1986, Evidence for the involvement of a UDP-glucose-dependent group translocator in sucrose uptake into vacuoles of storage roots of red beet, Planta 167: 410.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Margaret Thom
    • 1
  • Andrew Maretzki
    • 1
  1. 1.Hawaiian Sugar Planters’ AssociationAieaUSA

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