Mg2+-ATP-Dependent Glucose Transport in Tonoplast Vesicles of Normal and Agrobacterium Tumefaciens-Transformed Tobacco Cells
Agrobacterium tumefaciens-transformed tobacco cells are indenpendent of exogenous auxin due to the transcription of 2 T-DNA genes, which code for enzymes of auxin biosynthesis (Kemper et al., 1985; Van Onckelen et al., 1986). With primary potato tumors it has been shown that the active glucose uptake by transformed cells is drastically reduced by anti-auxins (Rausch et al., 1984). The inhibitory effect of anti-auxins as well as the modulating effects of chemicals influencing the cytoplasmic Ca2+ concentration (A 23187, verapamil) support the hypothesis that auxin modifies indeed active glucose uptake in transformed cells (Rausch, 1986 a and b). Both transformed and non-transformed cells of tobacco have now been further characterized with respect to the tonoplast localized proton/glucose antiport system.
KeywordsMicrosomal Membrane Auxin Biosynthesis Tonoplast Vesicle Sucrose Step Gradient Carrier Site
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