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Plant Vacuoles pp 287-289 | Cite as

Mg2+-ATP-Dependent Glucose Transport in Tonoplast Vesicles of Normal and Agrobacterium Tumefaciens-Transformed Tobacco Cells

  • Thomas Rausch
Part of the NATO ASI Series book series (NSSA, volume 134)

Abstract

Agrobacterium tumefaciens-transformed tobacco cells are indenpendent of exogenous auxin due to the transcription of 2 T-DNA genes, which code for enzymes of auxin biosynthesis (Kemper et al., 1985; Van Onckelen et al., 1986). With primary potato tumors it has been shown that the active glucose uptake by transformed cells is drastically reduced by anti-auxins (Rausch et al., 1984). The inhibitory effect of anti-auxins as well as the modulating effects of chemicals influencing the cytoplasmic Ca2+ concentration (A 23187, verapamil) support the hypothesis that auxin modifies indeed active glucose uptake in transformed cells (Rausch, 1986 a and b). Both transformed and non-transformed cells of tobacco have now been further characterized with respect to the tonoplast localized proton/glucose antiport system.

Keywords

Microsomal Membrane Auxin Biosynthesis Tonoplast Vesicle Sucrose Step Gradient Carrier Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Thomas Rausch
    • 1
  1. 1.Botanisches InstitutJ. W. Goethe-UniversitätFrankfurtGermany

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