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Plant Vacuoles pp 275-279 | Cite as

Proton-Motive Force and H+/Amino-Acid Antiport at the Tonoplast of Riccia Fluitans Rhizoid Cells, Revealed by Direct Probing with the pH-Sensitive Microelectrode

  • Eva Johannes
  • Hubert Felle
Part of the NATO ASI Series book series (NSSA, volume 134)

Abstract

The electrical properties of H+-amino-acid symport located at the plasmalemma of the freshwater liverwort Riccia fluitans has already been well characterized and described (Felle, 1981, 1983 and 1984; Johannes and Felle, 1985). Tracer compartment analysis proved fast and high accumulation of all amino-acids tested so far, including the nonmetabolizable amino-isobutyric acid (Aib) within the vacuole. It was desirable therefore, to carry out crucial tests, in order to clear up the mode of action of amino-acid transport across the tonoplast of this plant. It is generally assumed that a small electrical potential difference (positive inside the vacuole) and a large pH-gradient exist across tonoplast membranes. The recently developed turgor-resistent pH microelectrodes (Bertl and Felle, 1985; Felle and Bertl, 1986) seemed most promising (a) to measure the proton-motive force directly, and (b) to find out, whether amino-acids are transported along the proton-motive force or possibly in an electrically silent manner.

Keywords

Tonoplast Membrane Membrane Potential Difference Difference Trace Rhizoid Cell Electrogenic Proton Pump 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Eva Johannes
    • 1
  • Hubert Felle
    • 1
  1. 1.Botanisches Institut IJustus-Liebig-UniversitätGiessen, R.F.A.Germany

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