Plant Vacuoles pp 267-274 | Cite as

ATP-Dependent Uptake of Malate in Vacuoles from CAM Plant, Kalanchoe Daigremontiana

  • Kojiro Nishida
  • O. Tominaga
Part of the NATO ASI Series book series (NSSA, volume 134)


Intracellular translocation of malate during crassulacean acid metabolism (CAM) is a complex phenomenon. The key enzyme phosphoenolpyruvate carboxylase catalyzes the synthesis of malate in cytoplasm has been established (Kluge and Tin, 1978). In the dark period the malate synthesized in the cytoplasm is transported into vacuoles and accumulated in its. In the light period the malate is effluxed from the vacuoles into cytoplasm, and then deacidification proceed.


Malic Acid Crassulacean Acid Metabolism Small Vacuole Crassulacean Acid Metabolism Plant Protoplast Suspension 
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  1. Aebi, H., 1967, Catalase, in: “Methods of Enzymatic Analysis”, Vol. 1, H. U. Bermeyer, ed., Academic Press, New-York.Google Scholar
  2. Aoki, K., and Nishida, K., 1984, ATPase activity associated with vacuoles and tonoplast vesicles isolated from the CAM plant, Kalanchoe daigremontianum, Physiol. Plant., 60:21.CrossRefGoogle Scholar
  3. Arnon, D. I., 1949, Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris, Plant Physiol., 24:1.PubMedCrossRefGoogle Scholar
  4. Buser, C., and Matile, P., 1977, Malic acid in vacuoles isolated from Bryophyllum leaf cells, Z. Pflanzenphysiol., 82:462.Google Scholar
  5. Buser-Suter, C., Wiemken, A., and Matile, P., 1982, A malic acid permease in isolated vacuoles of a crassulacean acid metabolism plant, Plant Physiol., 69:456.PubMedCrossRefGoogle Scholar
  6. Hodges, T. K, and Leonard, R. T., 1974, Purification of a plasma-bound adenosinetriphosphatase from plant roots, in: Methods In Enzymology, 32:392.Google Scholar
  7. Kenyon, W. H., Kringstad, R., and Black, C. C., 1978, Diurnal changes in the malic acid content of vacuoles isolated from leaves of the crassulacean acid metabolism plant, FEBS Letters, 94:281.CrossRefGoogle Scholar
  8. Kluge, M., and Tin, I. P., 1978, “Crassulacean Acid Metabolism. Analysis of an Ecological Adaptation”, Springer-Verlag, Berlin.CrossRefGoogle Scholar
  9. Lüttge, U., Ball, E., and Tromballa, H. W., 1975, Potassium-independence of osmoregulated oscillations of malate2- levels in the cells of CAM leaves, Biochem. Physiol. Pflanzen, 167:267.Google Scholar
  10. Lüttge, U., and Ball, E., 1979, Electrochemical investigation of active malic acid transport at the tonoplast into the vacuoles of the CAM plant Kalanchoe daigremontianum, J. Membrane Biol., 47:401.CrossRefGoogle Scholar
  11. Lüttge, U., Smith, J. A. C., and Marigo, G., 1982, Membrane transport, osmoregulation and the control of CAM, in: “Crassulacean Acid Metabolism”, I. P. Tin and M. Gibbs, ed., Amer. Soc. Plant Physiol., Rockville, U.S.A.Google Scholar
  12. Nishida, K., and Hayashi, Y., 1979, Deacidification of the leaves of Bryophyllum calycinum under anaerobiosis. Abnormal efflux of malate into the cytoplasm, Plant Cell Physiol, 20, 1209–1215.Google Scholar
  13. Sato, F., Nishida, K., and Yamada, Y., 1980, Activities of carboxylation enzymes and products of 14CO2 fixation in photoautotrophically cultured cells, Plant Science Letters, 20:91.CrossRefGoogle Scholar
  14. Smith, J. A. C., Uribe, E. G., Ball, E., Heuer, S., and Lüttge, U., 1984, Characterization of the vacuolar ATPase activity of the crassulacean-acid-metabolism plant Kalanchoe daigremontianum Eur. J. Biochem., 141:415.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Kojiro Nishida
    • 1
  • O. Tominaga
    • 1
  1. 1.Botanical Institute, Faculty of SciencesKanazawa UniversityKanazawaJapan

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