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Isolation of Tonoplast Vesicles from Kiwi Fruit Cells: Electrochemical Investigation of K+ and H+ Membrane Transport Linked to a Magnesium-ATP Hydrolytic Activity

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Plant Vacuoles

Part of the book series: NATO ASI Series ((NSSA,volume 134))

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Abstract

Mechanical breaking of giant cells of the peripheral layer of Actinidia chinensis fruits produces in the extraction juice, many large vesicles that appear like free vacuoles or protoplasts (Chedhomme and Rona, 1984). The latter are of a much smaller size than the initial cells; these pseudoprotoplasts are always devoid of nuclei as in the case with “vacuoplasts” of Poterioochromonas malhamensis (Jochem et al., 1983).

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© 1987 Plenum Press, New York

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Rona, JP., Chedhomme, F., Convert, M., Monestiez, M. (1987). Isolation of Tonoplast Vesicles from Kiwi Fruit Cells: Electrochemical Investigation of K+ and H+ Membrane Transport Linked to a Magnesium-ATP Hydrolytic Activity. In: Marin, B. (eds) Plant Vacuoles. NATO ASI Series, vol 134. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5341-6_32

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  • DOI: https://doi.org/10.1007/978-1-4684-5341-6_32

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5343-0

  • Online ISBN: 978-1-4684-5341-6

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