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Plant Vacuoles pp 235-245 | Cite as

Isolation of Tonoplast Vesicles from Kiwi Fruit Cells: Electrochemical Investigation of K+ and H+ Membrane Transport Linked to a Magnesium-ATP Hydrolytic Activity

  • Jean-Pierre Rona
  • F. Chedhomme
  • M. Convert
  • Michèle Monestiez
Part of the NATO ASI Series book series (NSSA, volume 134)

Abstract

Mechanical breaking of giant cells of the peripheral layer of Actinidia chinensis fruits produces in the extraction juice, many large vesicles that appear like free vacuoles or protoplasts (Chedhomme and Rona, 1984). The latter are of a much smaller size than the initial cells; these pseudoprotoplasts are always devoid of nuclei as in the case with “vacuoplasts” of Poterioochromonas malhamensis (Jochem et al., 1983).

Keywords

Acer Pseudoplatanus Kiwi Fruit Vacuolar ATPase Plant Vacuole Tonoplast Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Jean-Pierre Rona
    • 1
  • F. Chedhomme
    • 1
  • M. Convert
    • 1
  • Michèle Monestiez
    • 1
  1. 1.Laboratoire d’Electrophysiologie des MembranesUniversité de Paris VIIParis-Cedex 05France

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