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Plant Vacuoles pp 219-225 | Cite as

Transport of Inorganic Ions in Tonoplast Vesicles

  • Ronald J. Poole
  • Eduardo Blumwald
Part of the NATO ASI Series book series (NSSA, volume 134)

Abstract

Since the tonoplast proton pumps create a positive membrane potential in the vacuole, the maintainance of high cytoplasmic K+ and the accumulation of Cl and NO3 in the vacuole requires only passive transport mechanisms (channels or uniports) for these ions, energized by ∆Ψ. On the other hand, the vacuolar accumulation of Na+ and Ca2+, and the retrieval of stored nutrient anions such as NO3 for metabolic use requires active transport mechanisms, which we now believe to be energized by proton efflux from the vacuole. This paper will discuss our work on these proton-coupled secondary transport mechanisms, with emphasis on their physiological significance, the technical problems involved, and the many questions remaining to be investigated.

Keywords

Acridine Orange Proton Leak Passive Permeability Active Transport Mechanism Tonoplast Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Blumwald, E., and Poole, R. J., 1985 a, Na+/H+ antiport in isolated tonolast vesicles from storage tissue of Beta vulgaris, Plant Physiol., 78: 163.PubMedCrossRefGoogle Scholar
  2. Blumwald, E., and Poole, R. J., 1985 b, Nitrate storage and retrieval in Beta vulgaris: Effects of nitrate and chloride on proton gradients in tonoplast vesicles, Proc. Natl. Acad. Sci. U.S.A., 82: 3683.PubMedCrossRefGoogle Scholar
  3. Blumwald, E., and Poole, R. J., 1986, Kinetics of Cat+/H+ antiport in isolated tonoplast vesicles from storage tissue of Beta vulgaris L., Plant Physiol., 80: 727.PubMedCrossRefGoogle Scholar
  4. Griffith, C. J., Rea, P. A., Blumwald, E., and Poole, R. J., 1986, Mechanism of stimulation and inhibition of tonoplast H+-ATPase of Beta vulgaris by chloride and nitrate, Plant Physiol., 81: 120.PubMedCrossRefGoogle Scholar
  5. Poole, R. J., Mehlhorn, R. J., and Packer, L., 1985, A study of transport in tonoplast vesicles using spin-labelled probes, in: “Biochemistry and Function of Vacuolar ATPase in Fungi and Plants”, B. P. Marin, ed., Springer-Verlag, Berlin, Heidelberg, New-York, Tokyo.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Ronald J. Poole
    • 1
  • Eduardo Blumwald
    • 1
  1. 1.Centre for Plant Molecular Biology, Biology DepartmentMcGill UniversityMontreal, P. Q.Canada

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