The Effects of Anions on PPi-Dependent Proton Transport in Tonoplast Vesicles from Oat Roots
The majority of investigations of anion transport at the tonoplast have involved examination of the effects of anions on ATP-dependent H+-transport in sealed tonoplast vesicles (e.g. Bennett and Spanswick, 1983; Churchill and Sze, 1984; Blumwald and Poole, 1985; Lew and Spanswick, 1985). These studies have shown that anions stimulate ΔpH formation and dissipate membrane potential (ΔΨ) and that these effects are sensitive to anion channel blockers such as DIDS and SITS. This is thought to indicate that anions move trough selective channels in the tonoplast in response to the Air generated by ATPase, causing dissipation of the ΔΨ and thus an increase in ΔpH, as expected from the chemiosmotic hypothesis (Mitchell, 1966).
KeywordsAnion Transport Storage Root Inorganic Pyrophosphatase Tonoplast Vesicle Avena Coleoptile
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