Plant Vacuoles pp 191-198 | Cite as

Mg, ATP-Dependent H+ Transport in Vacuoles and Tonoplast Vesicles from Acer Pseudoplatanus Cells

  • Roberta Colombo
  • Raffaela Cerana
  • Piera Lado
Part of the NATO ASI Series book series (NSSA, volume 134)


Large evidence has accumulated about the existence of an ATPase associated with the vacuolar membrane, with well-defined characteristics different from those of the plasmalemma ATPase. The tonoplast ATPase is stimulated by Cl, insensitive to monovalent cations, inhibited by nitrate and unaffected by vanadate and has a broad pH optimum between pH 6 and 8 (Sze, 1984; Marrè and Ballarin-Denti, 1985).


Osmotic Shock Acridine Orange Assay Medium Sodium Molybdate Tonoplast Vesicle 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Roberta Colombo
    • 1
  • Raffaela Cerana
    • 1
  • Piera Lado
    • 1
  1. 1.Centro C. N. R. per la Biologia Cellulare e Moleculare delle Piante, Dipartimento di Biologia “Luigi Gorini”Università di MilanoMilanoItaly

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