Generation of a pH-Gradient Across the Tonoplast of Vacuoles Isolated from Green Suspension Cells of Chenopodium Rubrum
Elucidation of solute transport across the tonoplast requires reliable information on how this membrane is energized. For this purpose we have studied ATP-dependent generation of the electrical membrane potential and conductance by means of the patch-clamp technique (Bentrup et al., 1985; Bentrup et al., 1986), and in parallel experiments, formation of the transtonoplast pH-gradient.
KeywordsAcridine Orange Acridine Orange Chenopodium Rubrum Leak Model Vacuolar Acidification
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- Bentrup, F.-W., Hoffmann, B., Gogarten-Boekels, M., Gogarten, J. P., and Baumann, C., 1985, A patch clamp study of tonoplast electrical properties in vacuoles isolated from Chenopodium rubrum suspension cells, Z. Naturf., 40 c:886.Google Scholar