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Plant Vacuoles pp 187-189 | Cite as

Generation of a pH-Gradient Across the Tonoplast of Vacuoles Isolated from Green Suspension Cells of Chenopodium Rubrum

  • Bernd Hoffmann
  • Margrit Gogarten-Boekels
  • Friedrich-Wilhelm Bentrup
Part of the NATO ASI Series book series (NSSA, volume 134)

Abstract

Elucidation of solute transport across the tonoplast requires reliable information on how this membrane is energized. For this purpose we have studied ATP-dependent generation of the electrical membrane potential and conductance by means of the patch-clamp technique (Bentrup et al., 1985; Bentrup et al., 1986), and in parallel experiments, formation of the transtonoplast pH-gradient.

Keywords

Acridine Orange Acridine Orange Chenopodium Rubrum Leak Model Vacuolar Acidification 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bentrup, F.-W., Hoffmann, B., Gogarten-Boekels, M., Gogarten, J. P., and Baumann, C., 1985, A patch clamp study of tonoplast electrical properties in vacuoles isolated from Chenopodium rubrum suspension cells, Z. Naturf., 40 c:886.Google Scholar
  2. Bentrup, F.-W., Hoffmann, B., Gogarten-Boekels, M., Gogarten, J. P., and Baumann, C., 1986, ATP-dependent acidification and tonoplast hyperpolarization in isolated vacuoles from green suspension cells of Chenopodium rubrum, Proc. Natl. Acad. Sci. U.S.A., 83: 2431.PubMedCrossRefGoogle Scholar
  3. Gogarten-Boekels, M., Gogarten, J. P., and Bentrup, F.-W., 1985, Kinetics and specificity of ATP-dependent proton translocation measured with acridine orange in microsomal fractions from green suspension cells of Chenopodium rubrum L., J. Plant Physiol., 118: 309.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Bernd Hoffmann
    • 1
  • Margrit Gogarten-Boekels
    • 1
  • Friedrich-Wilhelm Bentrup
    • 1
  1. 1.Botanisches Institut IJustus-Liebig-UniversitätGiessenGermany

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