Advertisement

Plant Vacuoles pp 179-186 | Cite as

Differential Susceptibilities of Tonoplast ATPase and PPase to Irreversible Inhibition by Chaotropic Anions

  • Philip A. Rea
  • Christoffer J. Griffith
  • Dale Sanders
Part of the NATO ASI Series book series (NSSA, volume 134)

Abstract

Isolated intact vacuoles and tonoplast vesicles are distinguished from other endomembranes by their possession of a NO 3 -inhibited, azide-insensitive ATPase (tp-ATPase; Rea et al., 1987). However, information concerning the mode of action of NO 3 is scant.

Keywords

Irreversible Inhibition Inorganic Pyrophosphatase Tonoplast Vesicle PPase Activity Chaotropic Anion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Griffith, C. J., Rea, P. A., Blumwald, E., and Poole, R. J., 1986, Mechanism of stimulation and inhibition of tonoplast H+-ATPase of Beta vulgaris by chloride and nitrate, Plant Physiol 81: 120.Google Scholar
  2. Hatefi, Y. H., and Hanstein, W. G., 1974, Destabilization of membranes by chaotropic anions, Methods In Enzymology 31: 770.Google Scholar
  3. Mandala, S., and Taiz, L., 1985, Partial purification of a tonoplast ATPase from corn coleoptiles, Plant Physiol 78: 327.PubMedCrossRefGoogle Scholar
  4. Manolson, M. F., Rea, P. A., and Poole, R. J., 1985, Identification of 3-O-(4-benzoyl)benzoyl-adenosine-5T-triphosphate-and N,N’-dicyclohexylcarbodiimide binding subunits of a higher plant H+-translocating tonoplast ATPase, J. Biol. Chem 260: 1 2273.Google Scholar
  5. Poole, R. J., Briskin, D. P., Kràtky, Z., and Johnstone, R. M., 1984, Density gradient localization of plasma membrane and tonoplast from storage tissue of growing and dormant red beet. Characterization of transport ATPase in tonoplast vesicles, Plant Physiol 74: 549.Google Scholar
  6. Rea, P. A., and Poole, R. J., 1985, Proton-translocating inorganic pyrophosphatase in red beet (Beta vulgaris L.) tonoplast vesicles, Plant Physiol 77: 46.Google Scholar
  7. Rea, P. A., and Poole, R. J., 1986, Chromatographic resolution of H+-translocating-pyrophoshatase from H+-translocating ATPase of higher plant tonoplast, Plant Physiol 81: 126.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Philip A. Rea
    • 1
  • Christoffer J. Griffith
    • 1
  • Dale Sanders
    • 1
  1. 1.Department of BiologyUniversity of YorkHeslington, YorkEngland, UK

Personalised recommendations