A Third-Category and a Fourth-Category H+-Phosphohydrolase at the Tonoplast
Most of the membranes of plant cells, including the plasma and Golgi membranes, are known to have H+ pumps but the tonoplast is the membrane which can be most easily isolated in the form of vesicles of low passive H+ conductance. Tonoplast vesicles constitute an experimental system in which the pH difference (∆pH) and electrical potential difference (∆ψ) between the two compartments separated by the vacuolar membrane can be measured and manipulated with comparative ease (Rea et al., 1986; Blumwald et al., 1987).
KeywordsVacuolar Membrane Chromaffin Granule Inorganic Pyrophosphatase Tonoplast Vesicle Radiation Inactivation
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