NBD-PC: A Tool to Study Phospholipase Activity in Protoplasts
Wild carrot cells can be grown in suspension culture so that they yield fusion permissive or fusogenic protoplasts (Boss et al., 1984). The presence of small vacuoles and high rates of inositol phospholipid metabolism were correlated with the high rates of fusion in these cells (Boss and Grimes, 1985). Increased phosphatidyl-inositol metabolism has been correlated with membrane fusion events such as secretory vesicle fusion with the plasma membrane (Laychock and Putney, 1982) and myoblast fusion during muscle maturation (Wakelam, 1983). Our hypothesis was that the rapid phosphatidylinositol headgroup metabolism found in the fusogenic protoplasts was indicative of a general increase in membrane lipid recycling as opposed to de novo synthesis and that increased lipid metabolism might contribute to the fusogenic state of the protoplasts.
KeywordsMyoblast Fusion Membrane Fusion Event Carrot Suspension Culture Carrot Suspension Culture Cell Carrot Protoplast
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