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Plant Vacuoles pp 135-141 | Cite as

Comparison of Two Methods for the Isolation of Vacuoles in Relation to Vacuolar ATPase Activity

  • Alain Pugin
  • Françoise Montrichard
  • Khanh Le-Quôc
  • Danièle Le-Quôc
Part of the NATO ASI Series book series (NSSA, volume 134)

Abstract

Several reports indicate that an anion-sensitive H+-ATPase insensitive to vanadate occurs in the tonoplast of higher plant cells (Lin et al., 1977; Leigh and Walker, 1980; Admon et al., 1981; Marin et al., 1981; Walker and Leigh, 1981; Aoki and Nishida, 1984; Smith et al., 1984; Mandala and Taiz, 1985). Our unability to measure an ATPase activity in vacuoles of Acer pseudoplatanus cells, prepared with DEAE-dextran and dextran sulfate as described by Alibert et al. (1982) lead us to incriminate the method used for their isolation. This hypothesis was supported by the unstability of the vacuoles out of the Ficoll used to form the density gradient. Therefore a new method for the isolation of vacuoles from Acer pseudoplatanus cells has been established. These vacuoles showed an ATPase activity, the properties of which have been studied and compared to those of other well known tonoplastic ATPases.

Keywords

ATPase Activity Dextran Sulfate Ammonium Molybdate Storage Root Large Vacuole 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Alain Pugin
    • 1
  • Françoise Montrichard
    • 1
  • Khanh Le-Quôc
    • 1
  • Danièle Le-Quôc
    • 1
  1. 1.Laboratoire de Biochimie, U.A. No 04531 Faculté des SciencesUniversité de Franche-ComtéBesançonFrance

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