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Plant Vacuoles pp 111-120 | Cite as

Analysis of the Electrical Fluctuations in Red Beet (Beta Vulgaris L.) Vacuoles

  • Jean-Paul Lassalles
  • Joël Alexandre
  • Michel Thellier
Part of the NATO ASI Series book series (NSSA, volume 134)

Abstract

Like animal cells, plant cells possess ionic pumps, such as ATPases, and ionic channels. The plant ATPases have been mainly studied by use of biochemical methods. Membrane fractions enriched with plasmalemma or with tonoplast have been prepared, and vesicles reconstituted from them. ATPases activities have been described, both on the plasmalemma and on the tonoplast. These activities are increased by MgATP. In the higher plants, the plasmalemma pump is strongly inhibited by vanadate, while the proton pump of the tonoplast is inhibited by NO3(Leigh, 1983; Marre and Ballarin-Denti, 1985). Besides the specific ionic effects, the ATPase activities are sensitive to the ionic strength of the bathing medium, which affects the surface electrostatic interactions in the membrane (Thibaud et al., 1984; Gibrat et al., 1985). In contrast with the active transports, the biochemical study of the passive ionic transports of the plant cells has practically not begun.

Keywords

ATPase Activity Noise Spectrum Bathing Medium Guard Cell Protoplast Specific Ionic Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Jean-Paul Lassalles
    • 1
  • Joël Alexandre
    • 1
  • Michel Thellier
    • 1
  1. 1.Laboratoire sur les Echanges CellulairesUnité Associée CNRSMont-Saint-AignanFrance

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