Metabolism of Lyso(bis)Phosphatidic Acid in Rabbit Alveolar Macrophages

  • Todd Thornburg
  • Felicia R. Cochran
  • Vicki L. Roddick
  • Moseley Waite
Part of the NATO ASI Series book series (NSSA, volume 133)


Lyso (bis)phosphatidic acid [L(bis)PA] is a phosphatidyl glycerol isomer with one fatty acid esterified to each glycerol moiety. This novel phospholipid was first identified by Body and Gray (1), and has since been found in a variety of mammalian tissues (2–5), normally comprising less than 1% of the total cellular lipid phosphorus. It appears that the stereochemistry of the lipid is unique, having the phosphoester bonds attached to the sn-1 carbons of each glycerol (6,7). The possible functional role of this lipid is only beginning to be understood.


Arachidonic Acid High Pressure Liquid Chromatography Phosphatidic Acid Phosphatidic Acid Glycerol Moiety 
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  1. 1.
    D. R. Body, and G. M. Gray, The isolation and characterization of phosphatidylglycerol and a structural isomer from pig lung, Chem. Phys. Lipids 1;254 (1967).CrossRefGoogle Scholar
  2. 2.
    C. F. Baxter, G. Rouser, and G. Simon, Variations among vertebrates of lung phospholipid class composition, Lipids 4:243 (1969).PubMedCrossRefGoogle Scholar
  3. 3.
    A. N. Siakotos, G. Rouser, and S. Fleisher, Isolation of highly purified human and bovine brain endothelial cells and nuclei and their phospholipid composition, Lipids 4:234 (1969).PubMedCrossRefGoogle Scholar
  4. 4.
    G. Rouser, G. Simon, and G. Kritchevsky, Species variations in phospholipid class distribution of organs. I. Kidney, liver and spleen, Lipids 4:599 (1969).PubMedCrossRefGoogle Scholar
  5. 5.
    G. Simon, and G. Rouser, Species variations in phospholipid class distribution of organs. II. Heart and skeletal muscle, Lipids 4:607 (1969).PubMedCrossRefGoogle Scholar
  6. 6.
    J. Brotherus, O. Renkonen, J. Herrman, and W. Fischer, Novel stereoconfiguration in lyso-bis-phosphatidic acid of cultured BHK-cells, Chem. Phys. Lipids 13:178 (1974).PubMedCrossRefGoogle Scholar
  7. 7.
    J. R. Wherrett, and S. Huterer, Bis-(monoacylglyceryl)-phosphate of rat and human liver: fatty acid composition and NMR spectroscopy, Lipids 8:531 (1973).PubMedCrossRefGoogle Scholar
  8. 8.
    G. Rouser, G. Kritchevsky, A. Yamamoto, and G. Simon, Accumulation of glycerophospholipid in classical Niemann-Pick disease, Lipids 3:287 (1968).PubMedCrossRefGoogle Scholar
  9. 9.
    J. R. Wherrett, and S. Huterer, Enrichment of bis-(monoacylglyceryl)-phosphate in lysosomes from rat liver, J. Biol. Chem. 247:4114 (1972).PubMedGoogle Scholar
  10. 10.
    J. Brotherus, and O. Renkonen, Subcellular distributions of lipids in cultured BHK cells: evidence of the enrichment of lysobis-phosphatidic acid and neutral lipids in lysosomes, J. lipid Res. 18:191 (1977).PubMedGoogle Scholar
  11. 11.
    R. J. Mason, T. P. Stossel, and M. Vaughan, Lipids of alveolar macrophages, polymorphonuclear leukocytes, and their phagocytic vesicles, J. Clin. Invest. 51:2399 (1972).PubMedCrossRefGoogle Scholar
  12. 12.
    S. Huterer, and J. Wherrett, Metabolism of bis(monoacylglycero)-phosphate in macrophages, J. Lipid Res. 20:966 (1979).PubMedGoogle Scholar
  13. 13.
    F. R. Cochran, V. Roddick, J. Connor, J. T. Thornburg, and M. Waite, Regulation of arachidonic acid metabolism in resident and BCG-activated alveolar macrophages: role of lyso(bis)-phosphatidic acid, J. Immunol., in press (1986).Google Scholar
  14. 14.
    B. J. H. M. Poorthuis, and K. Y. Hostetler, Studies on the localization and properties of bis(monoacylglyceryl)phosphate biosynthesis in rat liver, J. Biol. Chem. 251:4596 (1976).PubMedGoogle Scholar
  15. 15.
    Y. Matsuzawa, B. J. H. M. Poorthuis, and K. Hostetler, Mechanism of phosphatidylinositol stimulation of lysosomal bis(monoacylglyceryl) phosphate synthesis, J. Biol. Chem. 253:6650 (1978).PubMedGoogle Scholar
  16. 16.
    P. Somerharju, and O. Renkonen, Conversion of phosphatidylglycerol lipids to bis(monoacylglycero)phosphate in vivo, Biochim. Biophys. Acta 618:407 (1980).PubMedGoogle Scholar
  17. 17.
    M. Waite, V. Roddick, L. King, and F. Cochran, Conversion of phosphatidylglycerol to lyso(bis)phosphatidic acid by rabbit alveolar macrophages, J. Biol. Chem., submitted (1986).Google Scholar
  18. 18.
    F. Cochran, J. Connor, V. Roddick, and M. Waite, Lyso(bis)-phosphatidic acid: a novel source of arachidonic acid for oxidative metabolism by rabbit alveolar macrophages, Biochem. Biophys. Res. Commun. 130:800 (1985).PubMedCrossRefGoogle Scholar
  19. 19.
    G. H. deHaas, N. M. Postema, W. Nieuwenhuizen, and L. L. M. van Deenen, Purification and properties of phospholipase A from porcine pancreas, Biochim. Biophys. Acta 159:103 (1968).Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Todd Thornburg
    • 1
  • Felicia R. Cochran
    • 1
  • Vicki L. Roddick
    • 1
  • Moseley Waite
    • 1
  1. 1.Department of BiochemistryBowman Gray School of MedicineWinston-SalemUSA

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