Inositol Lipid Metabolism: A Topological Point of View
Since its discovery, the so-called “phospholipid effect” has deserved a great deal of investigations (for reviews see J. Williamson’s and K.W.A. Wirtz’s chapters in this book and ref.1–4), leading to the widely accepted scheme of inositol-1,4,5-trisphosphate (IP3) and diacylglyeerol (DG) production as second messengers of hormone action. As discussed elsewhere in this book, this dual pathway leads to increase the cytosolic free calcium concentration on one hand and to the activation of protein kinase C on the other. However, most of the studies described the overall effect of hormonereceptor interaction at the plasma membrane on the metabolic changes of inositol-phospholipids of the whole cell. It was then considered that inositol lipids were a homogeneous entity, ignoring possible subcellular compartmentation of metabolically different pools. The existence of microdomains on the plasma membrane is not documented either even if some data suggest the existence of agonist-linked pools. The aim of this chapter is to review the presently available data concerning the topology of inositollipid metabolism.
KeywordsHuman Platelet Cytosolic Phospholipase Human Erythrocyte Membrane Rabbit Platelet Inositol Lipid
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