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Bradykinin-Induced Inositol Phosphate Metabolism in Human A431 Epidermoid Carcinoma Cells

  • B. C. Tilly
  • P. A. van Paridon
  • I. Verlaan
  • K. W. A. Wirtz
  • S. W. de Laat
  • W. H. Moolenaar
Part of the NATO ASI Series book series (NSSA, volume 133)

Summary

Addition of the nonapeptide bradykinin to human A431 epidermoid carcinoma cells causes an immediate release of inositol phosphates and a rapid rise in [Ca2+]i. Half-maximal stimulation occurs at a bradykinin concentration of 4 nM. In the continuous presence of a saturating hormone concentration (1 μM) the inositol phosphate accumulation levels off within approximately 2 min; however, subsequent stimulation with histamine, another activator of phospholipase C, gives an additional increase in inositol phosphate production. Separation of the inositol phosphates by HPLC-anion exchange chromatography, reveals a rapid but transient accumulation of Ins(1,4,5)P3, followed by an increase in Ins (1,3,4,5)P4 and Ins(1,3,4)P3. Our data support a precursor/product-relationship between Ins(1,4,5)P3 and Ins(1,3,4)P3 with Ins(1,3,4,5)P4 as intermediate and argue against a messenger role of Ins(1,3,4,5)P4 and Ins(1,3,4)P3 in Ca2+ signalling.

Keywords

A431 Cell Inositol Phosphate Calcium Mobilization Inositol Phosphate Production Inositol Phosphate Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • B. C. Tilly
    • 1
  • P. A. van Paridon
    • 2
  • I. Verlaan
    • 1
  • K. W. A. Wirtz
    • 2
  • S. W. de Laat
    • 1
  • W. H. Moolenaar
    • 1
  1. 1.Hubrecht LaboratoryUtrechtThe Netherlands
  2. 2.Department of BiochemistryState University of UtrechtUtrechtThe Netherlands

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