Interaction of Trophoblastic Vesicles with Bovine Embryos Developing in Vitro
In many species, in vitro culture of fertilized embryos does not lead to normal cleavage and development as observed in vivo. When cultured in vitro, mouse eggs from outbred strains are blocked at the 2-cell stage unless they are transferred back into oviducts (Whittingham and Biggers, 1967). Hamster eggs also become blocked at this stage (Whittingham and Bavister, 1974) [see also Chapter 11 (Ed.)]. Most early bovine embryos (1- to 8-cell stage) arrest their development in vitro at the 8- to 12-cell stage (Thibault, 1966), in spite of a variety of culture media and conditions tested by several authors (reviewed by Wright and Bondioli, 1981).
KeywordsAlbumin Estrogen Lactate Electrophoresis Serotonin
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