Interaction of Trophoblastic Vesicles with Bovine Embryos Developing in Vitro
In many species, in vitro culture of fertilized embryos does not lead to normal cleavage and development as observed in vivo. When cultured in vitro, mouse eggs from outbred strains are blocked at the 2-cell stage unless they are transferred back into oviducts (Whittingham and Biggers, 1967). Hamster eggs also become blocked at this stage (Whittingham and Bavister, 1974) [see also Chapter 11 (Ed.)]. Most early bovine embryos (1- to 8-cell stage) arrest their development in vitro at the 8- to 12-cell stage (Thibault, 1966), in spite of a variety of culture media and conditions tested by several authors (reviewed by Wright and Bondioli, 1981).
KeywordsCorpus Luteum Coculture System Internal Fluid Bovine Embryo Morula Stage
Unable to display preview. Download preview PDF.
- Camous, S., Heyman, Y., and Ménézo, Y., 1984, In vitro cleavage of cow embryos and further survival assessment in vivo, Proceedings 10th Int. Congress on Anim. Reprod. and Artificial Insemination, Urbana, Vol. II, brief communication no. 221.Google Scholar
- Davis, D.L., and Day, B.N., 1978, Cleavage and blastocyst formation by pig eggs in vitro, J. Anim. Sci. 46: 1042–1053.Google Scholar
- Ilgreen, E.B., 1981, On the control of trophoblastic giant-cell transformation in the mouse: homotypic cellular interactions and polyploidy, J. Embryol. Exp. Morphol. 62: 183–202.Google Scholar
- Martal, J., Camous, S., Fèvre, J., Charlier, M., and Heyman, Y., 1984, Specificity of embryonic signals maintaining corpus luteum in early pregnancy in ruminants, Proceedings 10th Int. Congress on Anim. Reprod. and Artificial Insemination, Urbana, Vol. III, brief communication no. 510.Google Scholar
- Ménézo, Y., 1976, Milieu synthétique pour la survie et la maturation des gamètes et pour la culture de l’oeuf fécondé, C.R. Acad. Sci. D Paris 282: 1967–1970.Google Scholar
- Northey, D.L., and French, L.R., 1980, Effect of embryo removal and intrauterine infusion of embryonic homogenates on the lifespan of the bovine corpus luteum, J. Reprod. Fertil. 12: 539–550.Google Scholar
- Renard, J.P., du Mesnil du Buisson, F., Wintenberger-Torrès, S., and Ménézo, Y., 1976, In vitro culture of cow embryos from Day 6 and Day 7, in: Egg Transfer in Cattle (L.E.A. Rowson, ed.), Commission of the European Communities, Luxembourg, pp. 159–164.Google Scholar
- Seidel, G.E., Jr., 1977, Short maintenance and culture of embryos, in: Embryo Transfer in Farm Animals: A Review of Techniques and Applications (K.J. Betteridge, ed.), Canada Dept. of Agriculture, Monograph 16, pp 20-24.Google Scholar