Methods and Applications of Restriction Fragment Length Polymorphism Analysis to Plants

  • Benoit S. Landry
  • Richard W. Michelmore
Part of the Basic Life Sciences book series (BLSC, volume 41)


The lack of markers has hindered genetic analyses in many important plant species. Molecular biology now provides an opportunity to develop large numbers of genetic markers. Cloned DNA sequences can be used to probe specific regions of eukaryotic genomes for the presence of polymorphism at the DNA sequence level. This polymorphism is detected as variation in the length of DNA fragments homologous to a labeled probe after digestion of the genomic DNA with restriction endonucleases. Such variation has been termed restriction fragment length polymorphism (RFLP).


Restriction Fragment Length Polymorphism Human Leukocyte Antigen Class Polytene Chromosome Restriction Fragment Length Polymorphism Analysis Restriction Fragment Length Polymorphism Marker 


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  1. 1.
    Adams, J., and E.D. Rothman (1982) Estimation of phylogenetic relationships from DNA restriction patterns and selection of endonuclease sites. Proc. Natl. Acad. Sci., USA 79:3560–3564.PubMedCrossRefGoogle Scholar
  2. 2.
    Allard, R.W. (1956) Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24(10):235–278.Google Scholar
  3. 3.
    Amar, L., C.D. Arnaud, J. Cambrou, J.L. Guenet, and P.R. Avner (1985) Mapping the mouse X chromosome using random genomic probes and an interspecific mouse cross. EMBO J. 4:3695–3700.PubMedGoogle Scholar
  4. 4.
    Amasino, R.M. (1986) Acceleration of nucleic acid hybridization rate by polyethylene glycol. Analyt. Biochem. 152:304–307.Google Scholar
  5. 5.
    Antonarakis, S.E., C.D. Boehm, P.J.V. Giardina, and H.H. Kazazian, Jr. (1982) Nonrandom association of polymorphic restriction sites in the ß-globin gene cluster. Proc. Natl. Acad. Sci., USA 79:137–141.PubMedCrossRefGoogle Scholar
  6. 6.
    Arnheim, N., C. Strange, and H. Erlich (1985) Use of pooled DNA samples to detect linkage disequilibrium of polymorphic restriction fragments and human disease: Study of the HLA class II loci. Proc. Natl. Acad. Sci., USA 82:6970–6974.PubMedCrossRefGoogle Scholar
  7. 7.
    Ascione, R., N. Sacchi, D.K. Watson, R.J. Fisher, S. Fujiwara, A. Seth, and T.S. Papas (1986) Oncogenes: Molecular probes for clinical application in malignant diseases. Gene Analyt. Techn. 3:25–39.Google Scholar
  8. 8.
    Azen, E., K.M. Lyons, T. McGonigal, N.L. Barret, L.S. Clements, N. Maeda, E.F. Vanin, D.M. Carlson, and 0. Smithies (1984) Clones from the human gene complex coding for salivary proline-rich proteins. Proc. Natl. Acad. Sci., USA 81:5561–5565.PubMedCrossRefGoogle Scholar
  9. 9.
    Baker, B., J. Schell, H. Lorz, and N. Federoff (1986) Transposition of the maize controlling element “Activator” in tobacco. Proc. Natl. Acad. Sci., USA 83:4844–4848.PubMedCrossRefGoogle Scholar
  10. 10.
    Barker, D., M. Schafer, and R. White (1984) Restriction sites containing CpG show a higher frequency of polymorphism in human DNA. Cell 86:131–138.CrossRefGoogle Scholar
  11. 11.
    Bates, G.P., J.S. Cayenne, N.J. Lench, S.D.M. Brown, and R. Williamson (1985) Regional microdissection and microcloning from the short arm of chromosome 2. Cytogenet. Cell Genet. 40:578.Google Scholar
  12. 12.
    Bech-Hansen, N.T., P.S. Linsley, and D.W. Cox (1983) Restriction fragment length polymorphisms associated with immunoglobulin C-gamma genes reveal linkage disequilibrium and genomic organization. Proc. Natl. Acad. Sci., USA 80:6952–6956.PubMedCrossRefGoogle Scholar
  13. 13.
    Beckmann, J.S., and M. Soller (1983) Restriction fragment length polymorphisms in genetic improvement: Methodologies, mapping and costs. Theor. Appl. Genet. 67:35–43.Google Scholar
  14. 14.
    Beckmann, J.S., and M. Soller (1986) Restriction fragment length polymorphisms and genetic improvement of agricultural species. Euphytica 35:111–124.CrossRefGoogle Scholar
  15. 15.
    Bernatzky, R., and S.D. Tanksley (1986) Genetics of actin related sequences in tomato. Theor. Appl. Genet. 72:314–321.Google Scholar
  16. 16.
    Bernatzky, R., and S.D. Tanksley (1986) Majority of random cDNA clones correspond to single loci in the tomato genome. Mol. Gen. Genet. 203:8–14.Google Scholar
  17. 17.
    Bernatzky, R., and S.D. Tanksley (1986) Towards a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 112:887–898.PubMedGoogle Scholar
  18. 18.
    Blatt, C., K. Mileham, M. Haas, M.N. Nesbitt, M.E. Harper, and M.I. Simon (1983) Chromosomal mapping of the mink cell focus-inducing and xenotropic env gene family in the mouse. Proc. Natl. Acad. Sci., USA 80:6298–6302.PubMedCrossRefGoogle Scholar
  19. 19.
    Botstein, D., R.L. White, M. Skolnick, and R.W. Davis (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32:314–331.PubMedGoogle Scholar
  20. 20.
    Brown, W.M. (1980) Polymorphism in the mitochondrial DNA of humans as revealed by restriction endonuclease analysis. Proc. Natl. Acad. Sci., USA 77:3605–3609.PubMedCrossRefGoogle Scholar
  21. 21.
    Brown, W.R.A., and A.P. Bird (1986) Long-range restriction mapping of mammalian genomic DNA. Nature 322:477–481.PubMedCrossRefGoogle Scholar
  22. 22.
    Burr, B., S.V. Evola, F.A. Burr, and J.S. Beckmann (1983) The application of restriction fragment length polymorphism to plant breeding. In Genetic Engineering: Principles and Methods, Vol. 5, J.K. Setlow and A. Hollaender, eds. Plenum Press, New York, pp. 45–59.Google Scholar
  23. 23.
    Cann, R.L., W.M. Brown, and A.C. Wilson (1984) Polymorphic sites and the mechanism of evolution in human mitochondrial DNA. Genetics 106: 479–499.PubMedGoogle Scholar
  24. 24.
    Carle, G.F., and M.V. Olson (1985) An electrophoretic karyotype for yeast. Proc. Natl. Acad. Sci., USA 82:3756–3760.PubMedCrossRefGoogle Scholar
  25. 25.
    Carle, G.F., M. Frank, and M.V. Olson (1986) Electrophoretic separations of large DNA molecules by periodic inversion of the electric field. Science 232:65–68.PubMedCrossRefGoogle Scholar
  26. 26.
    Chakravarti, A., J.A. Philips, III, K.H. Mellits, K.H. Buetow, and P.H. Seeburg (1984) Patterns of polymorphism and linkage disequilibri-um suggest independent origins of the human growth hormone gene cluster. Proc. Natl. Acad. Sci., USA 81:6085–6089.PubMedCrossRefGoogle Scholar
  27. 27.
    Chardon, P., M. Kirszenbaum, C. Geffrotin, C. Renard, and D. Cohen (1985) Restriction fragment length polymorphism of the major histocompatibility complex of the pig. Immunogenetics 21:161–171.PubMedCrossRefGoogle Scholar
  28. 28.
    Chardon, P., M. Kirszenbaum, P.R. Cullen, C. Geffrotin, C. Auffray, J.L. Strominger, D. Cohen, and M. Vaiman (1985) Analysis of the sheep MHC using HLA class I, II, and C4 cDNA probes. Immunogenetics 22:349–358.PubMedCrossRefGoogle Scholar
  29. 29.
    Cheng, T.C., S.H. Orkin, S.E. Antonarakis, M.J. Potter, J.P. Sexton, A.F. Markam, P.J.V. Giardina, A. Li, and H.H. Kazazian, Jr. (1984) ß-Thalassemia in Chinese: Use of in vivo RNA analysis and oligonucleotide hybridization in systematic characterization of molecular defects. Proc. Natl. Acad. Sci., USA 81:2821–2825.PubMedCrossRefGoogle Scholar
  30. 30.
    Cox, G.N., S. Carr, J.M. Kramer, and D. Hirsh (1985) Genetic mapping of Caenorhabditis elegans collagen genes using DNA polymorphisms as phenotypic markers. Genetics 109:513–528.PubMedGoogle Scholar
  31. 31.
    Dahlberg, A.E., and E.J. Stellwag (1983) Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl paper. In Methods in Enzymology: Recombinant DNA, Part B, Vol. 100, R. Wu, L. Grossman, and K. Moldave, eds. Academic Press, Inc., New York, pp. 326–333.CrossRefGoogle Scholar
  32. 32.
    Davis, D.R., and C.A. Cullis (1982) A simple plant polytene chromosome system, and its use for in situ hybridization. Plant Mol. Biol. 1: 301–304.CrossRefGoogle Scholar
  33. 33.
    Davis, R.L., and N. Davidson (1984) Isolation of the Drosophila melanogaster dunce chromosomal region and recombinational mapping of dunce sequences with restriction site polymorphisms as genetic markers. Mol. Cell. Biol. 4:358–367.PubMedGoogle Scholar
  34. 34.
    Deumling, B. (1981) Sequence arrangement of a highly methylated satellite DNA of a plant, Scilla: A tandemly repeated inverted repeat. Proc. Natl. Acad. Sci., USA 78:338–342.PubMedCrossRefGoogle Scholar
  35. 35.
    Dracopoli, N.C., A.N. Houghton, and L.J. Old (1985) Loss of polymorphic restriction fragments in malignant melanoma: Implications for tumor heterogeneity. Proc. Natl. Acad. Sci., USA 82: 1470–1474.PubMedCrossRefGoogle Scholar
  36. 36.
    Drayna, D., K. Davies, D. Hartley, J.L. Mandel, G. Camerino, R. Williamson, and R. White (1984) Genetic mapping of the human X chromosome by using restriction fragment length polymorphisms. Proc. Natl. Acad. Sci., USA 81:2836–2839.PubMedCrossRefGoogle Scholar
  37. 37.
    Engels, W.R. (1981) Estimating genetic divergence and genetic variability with restriction endonucleases. Proc. Natl. Acad. Sci., USA 78:6329–6333.PubMedCrossRefGoogle Scholar
  38. 38.
    Evola, S.V., F.A. Burr, and B. Burr (1986) The suitability of restriction fragment length polymorphisms as genetic markers in maize. Theor. Appl. Genet. 71:765–771.CrossRefGoogle Scholar
  39. 39.
    Ewens, W.J., R.S. Spielman, and H. Harris (1981) Estimation of genetic variation at the DNA level from restriction endonuclease data. Proc. Natl. Acad. Sci., USA 78:3748–3750.PubMedCrossRefGoogle Scholar
  40. 40.
    Fisher, E.M.C., J.S. Cavanna, and S.D.M. Brown (1985) Microdissection and microcloning of the mouse X chromosome. Proc. Natl. Acad. Sci., USA 82:5846–5849.PubMedCrossRefGoogle Scholar
  41. 41.
    Gelderman, H. (1975) Investigations on inheritance of quantitative characters in animals by gene markers. I. Methods. Theor. Appl. Genet. 46:319–330.CrossRefGoogle Scholar
  42. 42.
    Giles, R.E., H. Blanc, H.M. Cann, and D.C. Wallace (1980) Maternal inheritance of human mitochondrial DNA. Proc. Natl. Acad. Sci., USA 77:6715–6719.PubMedCrossRefGoogle Scholar
  43. 43.
    Glaser, T., D. Gerhard, C. Payne, C. Jones, and D. Housman (1985) Three polymorphic DNA markers from chromosome 11. Cytogenet. Cell Genet. 40:643.Google Scholar
  44. 44.
    Griffing, B. (1956) A generalized treatment of the use of diallel crosses in quantitative inheritance. Heredity 10:31–50.CrossRefGoogle Scholar
  45. 45.
    Grodzicker, T., J. Williams, P. Sharp, and J. Sambrook (1974) Physical mapping of temperature-sensitive mutations of adenoviruses. Cold Spring Harbor Symp. Quant. Biol. 39:439–446.CrossRefGoogle Scholar
  46. 46.
    Gusella, J.F. (1986) DNA polymorphism and human disease. Ann. Rev. Biochem. 55:831–854.PubMedCrossRefGoogle Scholar
  47. 47.
    Gusella, J.F., C. Keys, A. Varsanyi-Breiner, F.T. Kao, C. Jones, T.T. Puck, and D. Housman (1980) Isolation and localization of DNA segments from specific human chromosomes. Proc. Natl. Acad. Sci., USA 77:28292833.Google Scholar
  48. 48.
    Helentjaris, T., and R. Gesteland (1983) Evaluation of random cDNA clones as probes for human restriction fragment polymorphisms. Mol. Appl. Genet. 2:237–247.Google Scholar
  49. 49.
    Helentjaris, T., D.F. Weber, and S. Wright (1986) Use of monosomics to map cloned DNA fragments in maize. Proc. Natl. Acad. Sci., USA (in press).Google Scholar
  50. 50.
    Helentjaris, T., G. King, M. Slocum, C. Siedenstrang, and S. Wegman (1985) Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol. Biol. 5(2):109–118.CrossRefGoogle Scholar
  51. 51.
    Helentjaris, T., M. Slocum, S. Wright, A. Schaefer, and J. Nienhuis (1986) Construction of linkage maps in maize and tomato using restriction fragment length polymorphism. Theor. Appl. Genet. (in press).Google Scholar
  52. 52.
    Holmes-Giannini, S., M. Schittini, J.S. Keithly, P.W. Warburton, C.R. Cantor, and L.H.T. Van der Ploeg (1986) Karyotype analysis of Leishmania species and its use in classification and clinical diagnosis. Science 232:762–765.CrossRefGoogle Scholar
  53. 53.
    Hudson, R.R. (1982) Estimating genetic variability with restriction endonucleases. Genetics 100:711–782.PubMedGoogle Scholar
  54. 54.
    Hutchison, J., A. Abbott, M. O’Dell, and R.B. Flavell (1985) A rapid screening technique for the detection of repeated DNA sequences in plant tissues. Theor. Appl. Genet. 69:329–333.CrossRefGoogle Scholar
  55. 55.
    Jeffreys, A.J., V. Wilson, and S.L. Thein (1985) Individual-specific “fingerprints” of human DNA. Nature 316:76–79.PubMedCrossRefGoogle Scholar
  56. 56.
    Kan, Y.W., and A.M. Dozy (1978) Antenatal diagnosis of sickle-cell anaemia by DNA analysis of amniotic fluid cells. Lancet 2:910–912.PubMedGoogle Scholar
  57. 57.
    Kao, F.T., J.A. Hartz, M.L. Law, and J.N. Davidson (1982) Isolation and chromosomal localization of unique DNA sequences from a human genomic library. Proc. Natl. Acad. Sci., USA 79:865–869.PubMedCrossRefGoogle Scholar
  58. 58.
    Kosambi, D.D. (1944) The estimation of map distance from recombination values. Ann. Eugen. 12:172–175.CrossRefGoogle Scholar
  59. 59.
    Krumlauf, R., M. Jeanpierre, and B.D. Young (1982) Construction and characterization of genomic libraries from specific human chromosomes. Proc. Natl. Acad. Sci., USA 79:2971–2975.PubMedCrossRefGoogle Scholar
  60. 60.
    Lalouel, J.M., T. Elsner, G.M. Lathrop, P. Callahan, A. Oliphant, R.L. White, D. Cohen, and J. Dausset (1985) The CEPH system of management of RFLP data. Cytogenet. Cell Genet. 40:676.Google Scholar
  61. 61.
    Landry, B.S., and R.W. Michelmore (1985) Selection of probes for restriction fragment length analysis from plant genomic clones. Plant Mol. Biol. Rep. 3(4):174–179.CrossRefGoogle Scholar
  62. 62.
    Lange, K., and M. Boehnke (1982) How many polymorphic marker genes will it take to span the human genome? Am. J. Hum. Genet. 34:842–845.PubMedGoogle Scholar
  63. 63.
    Law, M.L., J.N. Davidson, and F.T. Kao (1982) Isolation of a human repetitive sequence and its application to regional chromosome mapping. Proc. Natl. Acad. Sci., USA 79:7390–7394.PubMedCrossRefGoogle Scholar
  64. 64.
    Leonard, W.J., T.A. Donlon, R.V. Lebo, and W.C. Greene (1985) Localization of the gene encoding the human interleukin-2 receptor on chromosome 10. Science 228:1547–1549.PubMedCrossRefGoogle Scholar
  65. 65.
    Litt, M., and R.L. White (1985) A highly polymorphic locus in human DNA revealed by cosmid-derived probes. Proc. Natl. Acad. Sci., USA 82:6206–6210.PubMedCrossRefGoogle Scholar
  66. 66.
    Mather, K.M., and J.L. Jinks (1977) Introduction to Biometrical Genetics, Cornell University Press, New York, 231 pp.CrossRefGoogle Scholar
  67. 67.
    McClelland, M., L.G. Kessler, and M. Bittner (1984) Site-specific cleavage of DNA at 8- and 10-base-pair sequences. Proc. Natl. Acad. Sci., USA 81:983–987.PubMedCrossRefGoogle Scholar
  68. 68.
    Meinkoth, J., and G. Wahl (1984) Hybridization of nucleic acids immobilized on solid supports. Analyt. Biochem. 138:267–284.Google Scholar
  69. 69.
    Metzlaff, M., W. Troebner, F. Baldauf, R. Schegel, and J. Cullum (1986) Wheat specific repetitive sequences: Construction and characterization of four different genomic clones. Theor. Appl. Genet. 72: 207–210.Google Scholar
  70. 70.
    Miller, J.A. (1984) Diagnostic DNA. Science News 126:104–107.Google Scholar
  71. 71.
    Mullis, K.B., and F.A. Faloona (1986) Specific synthesis of DNA in vitro via a polymerase catalyzed chain reaction. In Methods in Enzymology, R. Wu, L. Grossman, and K. Moldave, eds. Academic Press, Inc., New York (in press).Google Scholar
  72. 72.
    Mullis, K.B., F.A. Faloona, S. Scharf, R. Saiki, G. Horn, and H. Erlich (1986) Specific enzymatic amplification of DNA in vitro: The polymerase chain reaction. Cold Spring Harbor Symp. Quant. Biol. Vol. 51 (in press).Google Scholar
  73. 73.
    Myers, R., Z. Larin, and T. Maniatis (1985) Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes. Science 230:1242–1246.PubMedCrossRefGoogle Scholar
  74. 74.
    Nasrallah, J.B., T.H. Kao, M.L. Goldberg, and M.E. Nasrallah (1985) A cDNA clone encoding an S-locus-specific glycoprotein from Brassica oleracea. Nature 318:263–267.CrossRefGoogle Scholar
  75. 75.
    Nienhuis, J., T. Helentjaris, M. Slocum, B. Ruggero, and A. Schaefer (1986) Restriction fragment length polymorphism analysis of loci associated with insect resistance in tomato. Crop Sci. (in press).Google Scholar
  76. 76.
    Nizetic, D., F. Figueroa, E. Nevo, and J. Klein (1985) Major histocompatibility complex of the mole-rat. Immunogenetics 22:55–67.PubMedCrossRefGoogle Scholar
  77. 77.
    O’Brien, S.J., ed. (1984) Genetic Maps, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 584 pp.Google Scholar
  78. 78.
    Orkin, S.H. (1983) The use of cloned DNA fragments to study human disease. In Genetic Engineering: Principles and Methods, Vol. 5, J.K. Setlow and A. Hollaender, eds. Plenum Press, New York, pp. 189–206.Google Scholar
  79. 79.
    Osborn, T.C., D.C. Alexander, and J.F. Fobes (1986) Identification of restriction fragment length polymorphism linked to genes controlling soluble solids content in tomato fruit. Theor. Appl. Genet. (submitted for publication).Google Scholar
  80. 80.
    Palmer, J.D., and D. Zamir (1982) Chloroplast DNA evolution and phylogenetic relationships in Lycopersicon. Proc. Natl. Acad. Sci., USA 79:5006–5010.PubMedCrossRefGoogle Scholar
  81. 81.
    Palmer, M., P.J. Wettstein, and J.A. Frelinger (1983) Evidence for extensive polymorphism of class I genes in the rat major histocompatibility complex (RT1). Proc. Natl. Acad. Sci., USA 80:7616–7620.PubMedCrossRefGoogle Scholar
  82. 82.
    Paulsen, K., G. Scherer, K.E. Davies, and H.H. Ropers (1985) The isolation of transcribed sequences from the human X chromosome. Cytogenet. Cell Genet. 40:721.Google Scholar
  83. 83.
    Petes, T.K., and D. Botstein (1977) Simple Mendelian inheritance of the reiterated ribosomal DNA of yeast. Proc. Natl. Acad. Sci., USA 74:5091–5095.PubMedCrossRefGoogle Scholar
  84. 84.
    Pichersky, E., R. Bernatzky, S. Tanksley, R.W. Breidenbach, A.P. Kaush, and A.R. Cashmore (1985) Molecular characterization and genetic mapping of two clusters of genes encoding chlorophyll a/b binding proteins in Lycopersicon esculentum. Gene 40:247–258.PubMedCrossRefGoogle Scholar
  85. 85.
    Pirrotta, V., H. Jackie, and J.E. Edstrom (1983) Microcloning of microdissected chromosome fragments. In Genetic Engineering: Principles and Methods, Vol. 5, J.K. Setlow and A. Hollaender, eds. Plenum Press, New York, pp. 1–17.Google Scholar
  86. 86.
    Polans, N.O., N.F. Weeden, and W.F. Thompson (1986) Distribution, inheritance and linkage relationships of ribosomal DNA spacer length variants in pea. Theor. Appl. Genet. 72:289–295.CrossRefGoogle Scholar
  87. 87.
    Polans, N.O., N.F. Weeden, and W.F. Thompson (1985) Inheritance, organization, and mapping of rbcS and cab multigene families in pea. Proc. Natl. Acad. Sci., USA 82:5083–5087.PubMedCrossRefGoogle Scholar
  88. 88.
    Potter, S., J. Newbold, C. Hutchison, III, and M. Edgell (1985) Specific cleavage analysis of mammalian mitochondrial DNA. Proc. Natl. Acad. Sci., USA 72:4496–4500.CrossRefGoogle Scholar
  89. 89.
    Reed, K.C., and D.A. Mann (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nucl. Acids Res. 13:7207–7221.CrossRefGoogle Scholar
  90. 90.
    Rick, C.M. (1975) The tomato. Handbook of Genetics 2:247–280.Google Scholar
  91. 91.
    Rivin, C.J., E.A. Zimmer, C.A. Cullis, V. Walbot, T. Huynh, and R.W. Davis (1983) Evaluation of genomic variability at the nucleic acid level. Plant Mol. Biol. Rep. 1:9–16.CrossRefGoogle Scholar
  92. 92.
    Rose, A.M., D.L. Bouillie, E.P.M. Condido, K.A. Beckenbach, and D. Nelson (1982) The linkage mapping of cloned restriction fragment length differences in Caenorhabditis elegans. Mol. Gen. Genet. 188: 286–291.PubMedCrossRefGoogle Scholar
  93. 93.
    Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen, and R.W. Allard (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Natl. Acad. Sci., USA 81:8014–8018.PubMedCrossRefGoogle Scholar
  94. 94.
    Saiki, R.K., S. Scharf, F. Faloona, K.B. Mullis, G.T. Horn, H.A. Erlich, and N. Arnheim (1985) Enzymatic amplification of 8-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354.PubMedCrossRefGoogle Scholar
  95. 95.
    Sax, K. (1923) The association of size differences with seed-coat pattern and pigmentation in Phaseolus vulgaris. Genetics 8:552–560.PubMedGoogle Scholar
  96. 96.
    Schwartz, C.E., and M.H. Skolnick (1985) The use of partial digestion of genomic DNA to reveal informative RFLPs. Cytogenet. Cell Genet. 40:740.Google Scholar
  97. 97.
    Sears, E.R. (1954) The aneuploids of common wheat. Un. Missoury Res. Bull. 572:1–59.Google Scholar
  98. 98.
    Sederoff, R.R., C.S. Levings, III, D.H. Timothy, and W.W.L. Hu (1981) Evolution of DNA sequence organization in mitochondrial genomes of Zea. Proc. Natl. Acad. Sci., USA 78:5953–5957.PubMedCrossRefGoogle Scholar
  99. 99.
    Singh, L., and K.W. Jones (1984) The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures. Nucl. Acids Res. 12(14):5627–5638.PubMedCrossRefGoogle Scholar
  100. 100.
    Soller, M., and J.S. Beckmann (1983) Genetic polymorphism in varietal identification and genetic improvement. Theor. Appl. Genet. 67:25–33.CrossRefGoogle Scholar
  101. 101.
    Soller, M., A. Genizi, and T. Brody (1976) On the power of experimental designs for the detection of linkage between marker loci and quantitative loci in crosses between inbred lines. Theor. Appl. Genet. 47:35–39.CrossRefGoogle Scholar
  102. 102.
    Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503–517.PubMedCrossRefGoogle Scholar
  103. 103.
    Stuber, C.W., H.H. Goodman, and R.H. Moll (1982) Improvement of yield and ear number resulting from selection at allozyme loci in a maize population. Crop Sci. 22:737–740.CrossRefGoogle Scholar
  104. 104.
    Suiter, K.A., J.F. Wendel, and J.S. Case (1983) Linkage-1: A pascal computer program for the detection and analysis of genetic linkage. J. Hered. 74:203–204.PubMedGoogle Scholar
  105. 105.
    Tanksley, S.D. (1983) Molecular markers in plant breeding. Plant Mol. Biol. Rep. 1:3–8.Google Scholar
  106. 106.
    Tanksley, S.D., H. Medina-Filho, and C.M. Rick (1982) Use of naturally-occurring enzyme variation to detect and map genes controlling quantitative traits in an interspecific backcross of tomato. Heredity 49:11–25.CrossRefGoogle Scholar
  107. 107.
    Thoday, J.M. (1961) Location of polygenes. Nature 191:368–370.CrossRefGoogle Scholar
  108. 108.
    Tsao, S., C. Brunk, and R. Pearlman (1981) Hybridizing dry agarose gels. Plant Mol. Biol. Newsletter 2(3):100–101.Google Scholar
  109. 109.
    Tsunewaki, K., and Y. Ogihara (1983) The molecular basis of genetic diversity among cytoplasms of Triticum and Aegilops species. II. On the origin of polyploid wheat cytoplasms as suggested by chloroplast DNA restriction fragment patterns. Genetics 104:155–171.PubMedGoogle Scholar
  110. 110.
    Vallejos, C.E., S.D. Tanksley, and R. Bernatzky (1986) Localization in the tomato genome of DNA restriction fragments containing sequences homologous to the rRNA (45S), the major chlorophyll a/b binding polypeptide and the ribulose bisphosphate carboxylase genes. Genetics 112:93–105.PubMedGoogle Scholar
  111. 111.
    Van der Ploeg, L.H.T., D.C. Schwartz, C.R. Cantor, and P. Borst (1984) Antigenic variation in Trypanosoma brucei analyzed by electrophoretic separation of chromosome-sized DNA molecules. Cell 37:77–84.PubMedCrossRefGoogle Scholar
  112. 112.
    Van der Ploeg, L.H.T., M. Smits, T. Ponnudurai, A. Vermeulen, J.H.E. Th. Meuwissen, and G. Langsley (1985) Chromosome-sized DNA molecules of Plasmodium falciparum. Science 229:658–661.PubMedCrossRefGoogle Scholar
  113. 113.
    Van Dilla, M.A., L.L. Deaven, K.L. Albrith, N.A. Allen, M.R. Aubuchon, M.F. Bartholdi, N.C. Brown, E.W. Campbell, A.V. Carrano, L.M. Clark, L.S. Cram, B.D. Crawford, J.C. Fuscoe, J.W. Gray, C.E. Hildebrand, P.J. Jackson, J.H. Jett, J.L. Longmire, C.R. Lozes, M.L. Luedemann, J.C. Martin, J.S. McNinch, L.J. Meincke, M.L. Mendelson, J. Meyne, R.K. Moyzis, A.C. Munk, J. Perlman, D.C. Peters, A.J. Silva, and B.J. Trask (1986) Human chromosome-specific DNA libraries: Construction and availability. Bio/Technology 4:537–552.CrossRefGoogle Scholar
  114. 114.
    Vogelstein, B., E.R. Fearon, S.R. Hamilton, and A.P. Feinberg (1985) Use of restriction fragment length polymorphisms to determine the clonal origin of human tumors. Science 227:642–645.PubMedCrossRefGoogle Scholar
  115. 115.
    Walbot, V., and R. Golberg (1979) Plant genome organization and its relationship to classical plant genetics. In Nucleic Acids in Plants, T. Hall and J. Davies, eds. CRC Press, Inc., Boca Raton, Florida, pp. 3–40.Google Scholar
  116. 116.
    Watkins, P.C., R.E. Tanzi, K.T. Gibbons, J.V. Tricoli, G. Landes, R. Eddy, T.B. Shows, and J.F. Gusella (1985) Isolation of polymorphic DNA segments from human chromosome 21. Nucl. Acids Res. 13:6075–6089.CrossRefGoogle Scholar
  117. 117.
    Welker, D.L., K.P. Hirth, P. Romans, A. Noegel, R.A. Firtel, and K.L. Williams (1986) The use of restriction fragment length polymorphisms and DNA duplications to study the organization of the actin multigene family in Dictyostelium discoideum. Genetics 112:27–42.PubMedGoogle Scholar
  118. 118.
    Willard, H.F. (1985) Polymorphic alpha satellite repeated DNA at the centromere of the X chromosome. Cytogenet. Cell Genet. 40:778.Google Scholar
  119. 119.
    Willard, H.F., M.H. Skolnick, P.L. Pearson, and J.L. Mandel (1985) Human gene mapping by recombinant DNA techniques. Cytogenet. Cell Genet. 40:360–489.CrossRefGoogle Scholar
  120. 120.
    Winoto, A., M. Steinmetz, and L. Hood (1983) Genetic mapping in the major histocompatibility complex by restriction enzyme site polymorphism: Most mouse class I genes map to the Tla complex. Proc. Natl. Acad. Sci., USA 80:3425–3429.PubMedCrossRefGoogle Scholar
  121. 121.
    Wong, Z., V. Wilson, A.J. Jeffreys, and S.L. Thein (1986) Cloning a selected fragment from a human DNA fingerprint: Isolation of an extremely polymorphic minisatellite. Nucl. Acids Res. 14(11):4605–4616.CrossRefGoogle Scholar
  122. 122.
    Yoder, J., F. Belzile, K. Alpert, J. Palys, and R.W. Michelmore (1986) Mobilization of the maize transposable element Ac in tomato. Tomato Biotechnology Symposium, Alan R. Liss, Inc., New York (in press).Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Benoit S. Landry
    • 1
  • Richard W. Michelmore
    • 1
  1. 1.Department of Vegetable CropsUniversity of CaliforniaDavisUSA

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