Genetic Tools for the Analysis of Gene Expression in Plants
The analysis of gene expression in plants is often based on correlations between data obtained by a variety of means: biochemical, physiological, and molecular genetic studies on the one hand, and classical genetic tools on the other hand. Among the techniques applied to the study of plant genes are screening of various genomic and complementary DNA (cDNA) libraries by synthetic oligonucleotides, gene- or organ-specific cDNAs, or antibody probes, in vitro translation of hybrid-released mRNAs, two-dimensional protein gel electrophoresis, immunoblotting, and transcript mapping (7,10). However, for an in depth understanding of how certain genes are regulated, alternative approaches are needed.
KeywordsGene Fusion Chimeric Gene Selectable Marker Gene Chloramphenicol Acetyl Transferase Target Plasmid
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