Expression of Sequences of Tobacco Mosaic Virus in Transgenic Plants and Their Role in Disease Resistance
Recently, it was reported that transgenic tobacco plants that express the coat protein gene of tobacco mosaic virus (TMV) exhibit a degree of resistance to infection by TMV (11). To determine whether the expression of other viral sequences would also confer resistance, transgenic plants were produced which expressed TMV nucleotides (nt) 3335 to 6395 (representing approximately 52% of the viral genome), their complement (antisense), or nucleotides that encode the 30-kDa protein. Plants that expressed the coat protein gene or other viral RNA sequences were inoculated with TMV and observed for development of disease symptoms. Symptoms were delayed only if transgenic plants expressed the coat protein gene; i.e., plants that express other sequences of TMV, of either the sense or antisense polarity, developed disease symptoms simultaneous with nontransgenic plants. The results indicate that the resistance to TMV infection is greater in transgenic plants that express the viral coat protein gene than in plants that express other sequences of TMV RNA.
KeywordsTransgenic Plant Transgenic Line Coat Protein Tobacco Mosaic Virus Transgenic Tobacco Plant
Unable to display preview. Download preview PDF.
- 2.Beachy, R.N., P. Abel, M.J. Oliver, B. De, R.T. Fraley, S.G. Rogers, and R.B. Horsch (1985) Potential for applying genetic transformation to studies of viral pathogenesis and cross-protection. In Biotechnology in Plant Science: Relevance to Agriculture in the Nineteen Eighties, M. Zaitlin, P. Day, and A. Hollaender, eds. Academic Press, Inc., New York, pp. 265–275.Google Scholar
- 3.Chen, Z.-L., M.A. Schuler, and R.N. Beachy (1986) Functional analysis of regulatory elements in a plant embryo-specific gene. Proc. Natl. Acad. Sci., USA Vol. 83 (in press).Google Scholar
- 6.Hamilton, R.I. (1980) Defenses triggered by previous invaders: Viruses. In Plant Disease, Vol. V, Academic Press, Inc., New York, pp. 279–303.Google Scholar
- 7.Maniatis, T., E.F. Fritsch, and J. Sambrook (1982) Molecular Cloning, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
- 9.Oliver, M.J., C.M. Deom, B.K. De,and R.N. Beachy (1986) In vitro transcription and translation of cloned cDNA encoding the 30-kDa protein gene of TMV. Virology Vol. 155 (in press).Google Scholar
- 10.Palukaitis, P., and M. Zaitlin (1984) A model to explain the “cross-protection” phenomenon shown by plant viruses and viroids. In Plant-Microbe Interactions. Vol. 1, T. Kosuge and E. Nester, eds. Macmillan Publishing Company, New York, pp. 420–429.Google Scholar
- 12.Rogers, S.G., K. O’Connell, R. Horsch, and R.T. Fraley (1986) Investigation of factors involved in foreign protein expression in transformed plants. In Biotechnology in Plant Science: Relevance to Agriculture in the Nineteen Eighties, M. Zaitlin, P. Day, and A. Hollaender, eds. Academic Press, Inc., New York, pp. 219–226.Google Scholar