Abstract
The advent of recombinant DNA technology has enabled the isolation of virtually any gene; indeed, the construction of complete gene libraries from any given organism is now routine. However, in many instances, it is extremely difficult to ascertain the biological function of a cloned gene sequence. Similarly, it is difficult to isolate a gene with a known phenotype for which there is no biochemical marker, e.g., a gene(s) affecting the shape or size of an organ.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bergmann, H., E. Preddie, and D.P.S. Verma (1983) Nodulin-35: A subunit of nodule-specific uricase (uricase II) induced and localized in uninfected cells. EMBO J. 2: 2333–2339.
Bevan, M. (1984) Binary Agrobacterium vectors for plant transformation. Nucl. Acids Res. 12: 8711–8721.
Coleman, J., P.J. Green, and M. Inouye (1984) The use of RNAs complementary to specific mRNAs to regulate the expression of individual bacterial genes. Cell 37: 429–436.
Fraley, R.T., S.G. Rogers, R.B. Horsch, D.A. Eichholtz, J.S. Flick, C.L. Flick, N.L. Hoffmann, and P.R. Sanders (1985) The SEV system: A new disarmed Ti plasmid vector system for plant transformation. Bio/Technology 3: 629–635.
Izant, J.G., and H. Weintraub (1984) Inhibition of thymidine kinase gene expression by anti-sense RNA: A molecular approach to genetic analysis. Cell 36: 1007–1015.
Jacobs, F.A., M. Zhang, M.G. Fortin, and D.P.S. Verma (1986) Several nodulins of soybean share structural domains but differ in their sub-cellular locations. Nucl. Acids Res. (in press).
Kim, S.K., and B.J. Wold (1985) Stable reduction of thymidine kinase activity in cells expressing high levels of anti-sense RNA. Cell 42: 129–138.
Klausner, A. (1985) Turning off unwanted genes with anti-RNA. Bio/-Technology 3: 763–764.
McGarry, T.J., and S. Lindquist (1986) Inhibition of heat shock protein synthesis by heat-inducible anti-sense RNA. Proc. Natl. Acad. Sci., USA 83: 399–403.
Melton, D.A. (1985) Injected anti-sense RNAs specifically block messenger RNA translation in vivo. Proc. Natl. Acad. Sci., USA 82: 144–148.
Mizuno, T., M.-Y. Chou, and M. Inouye (1984) A unique mechanism regulating gene expression: Translational inhibition by a complementary RNA transcript (micRNA). Proc. Natl. Acad. Sci., USA 81: 1966–1970.
Morelli, G., F. Nagy, R.T. Fraley, S.G. Rogers, and N.-H. Chua (1985) A short conserved sequence is involved in the light-inducibility of a gene encoding ribulose 1,5-biphosphate carboxylase small subunit of pea. Nature 315: 200–204.
Nguyen, T., M.G. Zelechowska, V. Foster, H. Bergmann, and D.P.S. Verma (1985) Primary structure of the soybean nodulin-35 gene encoding nodule-specific uricase localized in peroxisomes of uninfected cells of soybean. Proc. Natl. Acad. Sci., USA 80: 5040–5044.
Okayama, H., and P. Berg (1982) High-efficiency cloning of full-length cDNA. Mol. Cell. Biol. 2: 161–170.
Pestka, S., B.L. Dougherty, V. Jung, K. Hotta, and R.K. Pestka (1984) Anti-mRNA: Specific inhibition of translation of single mRNA molecules. Proc. Natl. Acad. Sci., USA 81: 7525–7528.
Rosenberg, U.B., A. Preiss, E. Seifert, H. Hackle, and D.C. Knipple (1985) Production of phenocopies by Kruppel anti-sense RNA injection into Drosophila embryos. Nature 313: 703–706.
Sengupta-Gopalan, C., J.W. Pitas, D.V. Thompson, and L.M. Hoffman (1986) Expression of host genes during root nodule development in soybeans. Mol. Gen. Genet. 203: 410–420.
Spencer, C.A., R.D. Gietz, and R.B. Hodgetts (1986) Overlapping transcription units in the dopa decarboxylase region of Drosophila. Nature 322: 279–281.
Verma, D.P.S., and K. Nadler (1984) Legume-Rhizobium-symbiosis: Host’s point of view. In Genes Involved in Microbe-Plant Interactions, D.P.S. Verma and T. Hohn, eds. Springer-Verlag, Wien and New York, pp. 57–93.
Verma, D.P.S., M.G. Fortin, J. Stanley, V.P. Mauro, S. Purohit, and N. Morrison (1986) Nodulins and nodulin genes of Glycine max. Plant Mol. Biol. 7: 51–61.
Weintraub, H., J.G. Izant, and R.M. Harland (1985) Anti-sense RNA as a molecular tool for genetic analysis. Trends in Genetics 1: 22–25.
Williams, T., and M. Fried (1986) A mouse locus at which transcription from both DNA strands produces mRNAs complementary at their 3’ ends. Nature 322: 275–279.
Wong, S.L., and D.P.S. Verma (1985) Promoter analysis of a soybean nuclear gene coding for nodulin-23, a nodule-specific polypeptide involved in symbiosis with Rhizobium. EMBO J. 4: 2431–2438.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Plenum Press, New York
About this chapter
Cite this chapter
Verma, D.P.S., Delauney, A.J., Nguyen, T. (1987). A Strategy towards Antisense Regulation of Plant Gene Expression. In: Bruening, G., Harada, J., Kosuge, T., Hollaender, A., Kuny, G., Wilson, C.M. (eds) Tailoring Genes for Crop Improvement. Basic Life Sciences, vol 41. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5329-4_13
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5329-4_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5331-7
Online ISBN: 978-1-4684-5329-4
eBook Packages: Springer Book Archive