Ion Channels in T Lymphocytes
Part of the
Advances in Experimental Medicine and Biology
book series (AEMB, volume 213)
Ion channels, proteins that gate the flux of ions across the cell membrane, control an impressive array of physiological processes, including conduction of nerve impulses, synaptic transmission, hormone secretion, generation of the heart beat, initiation of muscle contraction, and transduction of sensory stimuli. To a large extent, this diversity of functions is a reflection of the diversity of ion channel types. Several distinct channel types have been characterized in neurons using a variety of electrophysiological techniques (Table 1; see also ref. 1). In the past several years, some of the same channel types have been observed in cells outside the nervous system, raising the possibility that ion channels may serve functions in electrically non-excitable tissues that are quite distinct from their electrical activities in the nervous system. In fact, a collection of direct and indirect observations suggests that ion channels similar to those found in nerve and muscle participate in the control of biological events associated with cellular proliferation and cytokine production that are crucial to the functioning of the immune system.
KeywordsMembrane Potential Channel Blocker Sodium Channel Potassium Channel Channel Expression
B. Hille. Ionic channels of excitable membranes. Sinauer Associates, Sunderland Mass., (1984).Google Scholar
M. H. Freedman, M. C. Raff, and B. Gomperts. Induction of increased calcium uptake in mouse T lymphocytes by concanavalin A and its modulation by cyclic nucleotides. Nature
255:378, (1978).ADSCrossRefGoogle Scholar
R. Y. Tsien, T. Pozzan, and T. J. Rink. T-cell mitogens cause early changes in cytoplasmic free Ca2+
and membrane potential in lymphocytes. Nature
295:68, (1982).ADSCrossRefGoogle Scholar
T. R. Hesketh, G. A. Smith, J. P. Moore, M. V. Taylor, and J. C. Metcalfe. Free cytoplasmic calcium concentration and the mitogenic stimulation of lymphocytes. J. Biol. Chem.
258:4876, (1983).Google Scholar
A. Weiss, J. Imboden, D. Shoback, and J. Stobo. Role of T3 surface molecules in human T cell activation: T3-dependent activation results in an increase in cytoplasmic free calcium. Proc. Natl. Acad. Sci. USA
81:4149, (1984).ADSCrossRefGoogle Scholar
E. Nisbet-Brown, R. K. Cheung, J. W. W. Lee, and E. W. Gelfand. Antigen-dependent increase cytosolic free calcium in specific human T-lymphocyte clones. Nature
316:545, (1985).ADSCrossRefGoogle Scholar
R. B. Whitney, and R. M. Sutherland. Characteristics of calcium accumulation by lymphocytes and alterations in the process induced by phytohemagglutinin. J. Cell. Physiol.
82:9, (1973).CrossRefGoogle Scholar
D. L. Birx, M. Berger, and T. A. Fleisher. The interference of T cell activation by calcium channel blocking agents. J. Immunol.
133:2904, (1974).Google Scholar
V. C. Maino, N. M. Green, and M. J. Crumpton. The role of calcium ions in initiating transformation of lymphocytes. Nature
251:324, (1974).ADSCrossRefGoogle Scholar
A. Mastro, and M. C. Smith. Calcium dependent activation of lymphocytes by ionophore A23187 and a phorbol ester tumor promoter. J. Cell. Physiol.
116:51, (1983).CrossRefGoogle Scholar
J. C. Metcalfe, T. Pozzan, G. A. Smith, and T. R. Hesketh. A calcium hypothesis for the control of cell growth. Biochem. Soc. Svmp.
45:1, (1980).Google Scholar
G. B. Segel, W. Simon, and M. A. Lichtman. Regulation of sodium and potassium transport in phytohemagglutinin-stimulated human blood lymphocytes. J. Clin-Invest.
64:834, (1979).CrossRefGoogle Scholar
P. E. R. Tatham, and P. J. Delves. Flow cytometric detection of membrane potential changes in murine lymphocytes induced by concanavalin A. Biochem. J.
221:137, (1984).Google Scholar
V. L. Lew, and H. G. Ferreira. Calcium transport and the properties of a calcium-activated potassium channel in red cell membranes. Curr. Top. Membr. Trans.
10:217, (1978).CrossRefGoogle Scholar
T. E. DeCoursey, K. G. Chandy, S. Gupta, and M. D. Cahalan. Voltage-gated K+
channels in human T lymphocytes: a role in mitogenesis? Nature
307:465, (1984).ADSCrossRefGoogle Scholar
T. E. DeCoursey, K. G. Chandy, S. Gupta, and M. D. Cahalan. Voltage-dependent ion channels in T-lymphocytes. J. Neuroimmunol.
10:71, (1985).CrossRefGoogle Scholar
L. M. Hondeghem, and B. G. Katzung. Time- and voltage-dependent interactions of antiarrhythmic drugs with cardiac sodium channels. Biochim. et Biophvs. Acta
472:373, (1977).CrossRefGoogle Scholar
O. P. Hamill, A. Marty, E. Neher, B. Sakmann, and F. J. Sigworth. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch
. 391:85, (1981).CrossRefGoogle Scholar
Y. Fukushima, S. Hagiwara, and R. E. Saxton. Variation of calcium current during the cell growth cycle in mouse hybridoma lines secreting immunoglobulins. J. Physiol.
355:313. (1984).Google Scholar
D. L. Ypey, and D. E. Clapman. Development of a delayed outward-rectifying K+
conductance in cultured mouse peritoneal macrophages. Proc. Natl. Acad. Sci. USA
81:3083, (1984).ADSCrossRefGoogle Scholar
E. K. Gallin, and P. A. Sheehy. Differential expression of inward and outward potassium currents in the macrophage-like cell line J774.1. J. Physiol.
369:475, (1985).Google Scholar
M. D. Cahalan, K. G. Chandy, T. E. DeCoursey, and S. Gupta. A voltage-gated potassium channel in human T lymphocytes. J. Physiol.
358:197, (1985).Google Scholar
K. G. Chandy, T. E. DeCoursey, M. D. Cahalan, C. McLaughlin, and S. Gupta. Voltage-gated K channels are required for T lymphocyte activation. J. Exp. Med.
160:369, (1984).CrossRefGoogle Scholar
B. Sharma and P. I. Terasaki. In vitro
immunization to cultured tumor cells. Cancer Res
. 34:115. (1974).Google Scholar
D. R. Matteson and C. Deutsch. K channels in T lymphocytes: a patch clamp study using monoclonal antibody adhesion. Nature
307:468, (1984).ADSCrossRefGoogle Scholar
Y. Fukushima, S. Hagiwara, and M. Henkart. Potassium current in clonal cytotoxic lymphocytes from the mouse. J. Physiol.
351:645, (1984).Google Scholar
K. S. Lee and R. W. Tsien. Mechanism of calcium channel blockade by verapamil, D600, diltiazem, and nitrendipine in single dialysed heart cells. Nature
302:790, (1983).ADSCrossRefGoogle Scholar
P. Bregestovski, A. Redkozubov, and A. Alexeev. Elevation of intracellular calcium reduces voltage-dependent potassium conductance in human T cells. Nature
319:776, (1986).ADSCrossRefGoogle Scholar
T. E. DeCoursey, K. G. Chandy, M. Fischbach, N. Talal, S. Gupta, M. D. Cahalan. Two types of K channels in T lymphocytes from MRL mice. Biophvs. J.
47:387a, (1985).Google Scholar
T. E. DeCoursey, K. G. Chandy, S. Gupta, and M. D. Cahalan. Two types of potassium channels in murine T lymphocytes. J. Gen. Physiol.
(in press).Google Scholar
E. K. Gallin. Electrophysiological properties of macrophages. Fed. Proc.
43:2385, (1984).ADSGoogle Scholar
S. Ikeda and F. Weight. Inward rectifying K+
currents recorded from rat basophilic leukemic cells by whole cell patch clamp. Neurosci. Abstr.
10:870, (1984).Google Scholar
L. Schlichter, N. Sidell, and S. Hagiwara. Potassium channels mediate killing by human natural killer cells. Proc. Natl. Acad. Sci. USA
83:451, (1986).ADSCrossRefGoogle Scholar
J. H. Russell and C. B. Dobos. The role of monovalent cations in the interaction between the cytotoxic T lymphocyte and its target. Eur. J. Immunol.
11:840, (1981).CrossRefGoogle Scholar
W. Schwartz and H. A. Kolb. Voltage-dependent kinetics of an anionic channel of large unit conductance in macrophages and myotube membranes. Pflugers Arch
. 402:281, (1984).CrossRefGoogle Scholar
M. E. Krouse, G. T. Schneider, and P. W. Gage. A large anion-selective channel has seven conductance levels. Nature
319:58, (1986).ADSCrossRefGoogle Scholar
P. T. A. Gray and J. M. Ritchie. Ion channels in Schwann and glial cells. TINS
9:411, (1986).Google Scholar
M. M. Bosma. Chloride channels in neoplastic B lymphocytes. Biophvs. J.
49:413a, (9186).Google Scholar
C. Deutsch, D. Krause, and S. C. Lee. Voltage-gated potassium conductance in human T lymphocytes stimulated with phorbol ester. J. Physiol.
372:405, (1986).Google Scholar
S. Lee, D. Krause, and C. Deutsch. Increased voltage-gated K+
conductance in T-lymphocytes stimulated with phorbol ester. Biophvs. J.
47:147a, (1985).Google Scholar
T. E. DeCoursey, K. G. Chandy, M. Fischbach, N. Talal, S. Gupta, and M. D. Cahalan. Potassium channel expression in proliferating murine T lymphocytes. Fed. Proc.
44:1310, (1985).Google Scholar
T. E. DeCoursey, K. D. Chandy, S. Gupta, and M. D. Cahalan. Mitogen induction of ion channels in murine T lymphocytes. J. Gen. Physiol.
(in press).Google Scholar
S. C. Lee, D. E. Sabath, C. Deutsch, and M. B. Prystowsky. Increased voltage-gated potassium conductance during interleukin 2-stimulated proliferation of a mouse helper T lymphocyte clone. J. Cell. Biol.
102:1200, (1986).CrossRefGoogle Scholar
R. S. Lewis, K. G. Chandy, S. Gupta, and M. D. Cahalan. Changes in K channel expression during the life cycle of murine T lymphocytes. Neurosci Abstr
. in press, (1986).Google Scholar
K. G. Chandy, T. E. DeCoursey, M. Fischbach, N. Talal, M. D. Cahalan, and S. Gupta. Altered K+
channel expression in abnormal T lymphocytes from mice with the lpr
gene mutation. Science
233:1197, (1986).ADSCrossRefGoogle Scholar
G. B. Mills, R. K. Cheung, S. Grinstein, and E. W. Gelfand. Increase in cytosolic free calcium concentration is an intracellular messenger for the production of interleukin 2 but not for the expression of the interleukin 2 receptor. J. Immunol.
134:1640, (1985).Google Scholar
E. W. Gelfand, R. W. Cheung, and S. Grinstein. Role of membrane potential in the regulation of lectin-induced calcium uptake. J. Cell. Physiol.
121:533, (1984).CrossRefGoogle Scholar
E. D. Murphy. Lymphoproliferation (lpr
) and other single locus models for murine lupus. In Immunologic Defects in Laboratory Animals. M. E. Gershwin, B. Merchant eds. Plenum Press, New York, (1981) Vol 2 pp. 143.CrossRefGoogle Scholar
A. N. Theofilopoulos, R. A. Eisenberg, M. Bourdon, J. S. Crowell, and F. J. Dixon. Distribution of lymphocytes identified by surface markers in murine strains with systemic lupus erythematosus-like syndromes. J. Exp. Med.
149:516, (1979).CrossRefGoogle Scholar
D. E. Lewis, J. V. Giorgi, and N. L. Warner. Flow cytometry analysis of T cells and continuous lines from autoimmune MRL/l mice. Nature
289:298, (1981).ADSCrossRefGoogle Scholar
H. C. Morse, W. F. Davidson, R. A. Yetter, E. D. Murphy, J. B. Roths, and R. L. Coffman. Abnormalities induced by the mutant gene lpr
: expansion of a unique lymphocyte subset. J. Immunol.
129:2612, (1982).Google Scholar
F. J. Dumont, R. C. Habbersett, E. A. Nichols, J. A. Treffinger, and A. S. Tung. A monoclonal antibody (100C5) to the Lyt-2-
T cell population expanding in MRL/MpJ-lpr/lpr
mice detects a surface antigen normally expressed on Lyt-2+
cells and B cells. Eur. J. Immunol.
13:455, (1983).CrossRefGoogle Scholar
F. J. Dumont, R. C. Habbersett, and E. A. Nichols. A new lymphocyte surface antigen defined by a monoclonal antibody (9F3) to the T cell population expanding in MRL/MpJ-lpr/lpr
mice. J. Immunol.
133:809, (1984).Google Scholar
F. Takei. Unique surface phenotype of T cells in lymphoproliferative autoimmune MRL/Mp-lpr/lpr
mice. J. Immunol.
133:1951, (1984).Google Scholar
H. S. Oettgen, C. Terhorst, L. C. Cantley, and P. M. Rosoff. Stimulation of the T3-T cell receptor complex induces a membrane-potential-sensitive calcium influx. Cell
40:583, (1985).CrossRefGoogle Scholar
Y. Fukushima and S. Hagiwara. Voltage-gated Ca2+
channel in mouse myeloma cells. Proc. Natl. Sci. USA
80:2240, (1983).ADSCrossRefGoogle Scholar
Y Fukushima and S. Hagiwara. Currents carried by monovalent cations through calcium channels in mouse neoplastic B lymphocytes. J. Physiol.
358:255, (1985).Google Scholar
E. K. Gallin. Calcium- and voltage-activated potassium channels in human macrophages. Biophys. J.
46:821, (1984).ADSCrossRefGoogle Scholar
D. Nelson, E. R. Jacobs, J. M. Tang, J. M. Zeller, and R. C. Bone. Immunoglobulin G induces single channels in human alveolar macrophage membranes. J. Clin. Invest.
76:500, (1985).CrossRefGoogle Scholar
© Plenum Press, New York 1987