Human T Lymphocyte Activation
The T lymphocyte receptor for antigen and histocompatibility molecules is a molecular complex comprised of five polypeptide chains. Both the 49KD alpha and 43KD beta chains are immunoglobulin-like and thus contain variable domains responsible for ligand binding. In contrast, the 20–25KD T3 gamma, delta and epsilon chains are monomorphic structures presumably involved in transmembrane signalling. The alpha and beta subunits are disulfide bonded to each other and held in noncovalent association with the T3 chains. T3-Ti receptor crosslinking leads to conformational modification of a second T lineage specific molecule, termed the 50KD T11 structure and in turn, protein kinase C activation, elevation in intracytoplasmic free calcium and Na+/H+ antiport stimulation.
KeywordsMajor Histocompatibility Complex Phorbol Ester Antigen Receptor Beta Subunit Clonal Proliferation
Unable to display preview. Download preview PDF.
- 2.R. M. Zinkernagel, and P. C. Doherty. H-2 compatibility requirement for T cell mediated lysis of target cells infected with lymphocytic choiromeningitis virus; different cytotoxic T cell specificities are associated with structures coded for in either H-2KI or H-2D. J. Exp. Med. 141:1427, (1975).CrossRefGoogle Scholar
- 5.J. M. Kanellopoulos, N. M. Wigglesworth, M. J. Owen, and M. J. Crumpton. Biosynthesis and molecular nature of the T3 antigen of human T lymphocyte. EMBO J. 2:1807, (1983).Google Scholar
- 7.S. C. Meuer, R. E. Hussey, D. A. Cantrell, J. C. Hodgdon, S. F. Schlossman, K. A. Smith, and E. L. Reinherz. Triggering of the T3-Ti antigen receptor complex results in clonal T cell proliferation via an interleukin 2 dependent autocrine pathway. Proc. Natl. Acad. Sci. USA 81:1509, (1984).ADSCrossRefGoogle Scholar
- 8.J. M. Williams, D. Deloria, J. A. Hansen, C. A. Dinarello, R. Loertscher, H. M. Shapiro, and T. B. Strom. The events of primary T cell activation can be stage by use of Sepharose-bound anti-T3 (64.1) monoclonal antibody and purified interleukin 1. J. Immunol. 135:2249. (1985).Google Scholar
- 14.H. D. Royer, O. Acuto, M. Fabbi, R. Tizard, K. Ramachandran, J. Smart, and E. L. Reinherz. Genes encoding the Ti beta subunit of the antigen/MHC receptor undergo rearrangement at a precise stage of intrathymic ontogeny and preceding surface expression of the T3-Ti molecular complex. Cell 39:261, (1984).CrossRefGoogle Scholar
- 16.D. A. Fox, R. E. Hussey, K. A. Fitzgerald, A. Bensussan, J. F. Daley, S. F. Schlossman, and E. L. Reinherz. Activation of human thymocytes via the 50KD T11 sheep erythrocyte binding protein induces the expression of interleukin 2 receptors on both T3+ and T3- populations. J. Immunol. 134:330, (1985).Google Scholar
- 19.A. Alcover, M. J. Weiss, J. F. Daley, and E. L. Reinherz. The T11 surface glycoprotein is functionally linked to a calcium channel in precursor and mature T lineage cells. Proc. Natl. Acad. Sci. USA (in press).Google Scholar
- 21.A. Alcover, C. Milanese, and E. Reinherz. Role of Na+/H+ exchanger in the activation of human lymphocytes. (Submitted).Google Scholar