Early Events in Lymphocyte Activation Triggered Via CD3/Ti or CD2

  • P. C. L. Beverley
  • D. L. Wallace
  • K. O’Flynn
  • D. C. Linch
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 213)


In recent years considerable insight has been gained into the sequence of events necessary for activation of lymphocytes. Several techniques have contributed to this progress; in particular the use of monoclonal cell populations, monoclonal antibodies to defined surface molecules and novel assays for detecting early events consequent on ligand binding has allowed the definition of several surface molecules of lymphocytes which can transmit signals to the cell and a variety of biochemical events which occur following ligand binding. Nevertheless many questions remain unresolved.


Phorbol Myristate Acetate Phorbol Myristate Acetate Accessory Cell Peripheral Blood Mononuclear Follow Ligand Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J. Van Wauwe, J. De Mey, and J. Goossens. OKT3: a monoclonal anti-human T-lymphocyte antibody with potent mitogenic properties. J. Immunol. 124:2708, (1980).Google Scholar
  2. 2.
    S. C. Meuer, R. E. Hussey, M. Fabbi, D. Fox, O. Acuto, K. A. Fitzgerald, J. C. Hodgdon, J. P. Protentis, S. F. Schlossman, and E. L. Reinherz. An alternative pathway of T-cell activation: a functional role for the 50 KId T11 sheep erythrocyte receptor protein. Cell 36:897, (1984).CrossRefGoogle Scholar
  3. 3.
    A. Moretta, G. Pantaleo, M. Lopez-Boter, and L. Moretta. Involvement of T44 molecules in an antigen-independent pathway of T cell activation. Analysis of the correlations to the T cell antigen-receptor complex. J. Exp. Med. 162:823, (1985).CrossRefGoogle Scholar
  4. 4.
    K. O’Flynn, M. Russul-Saib, I. Ando, D. L. Wallace, P. C. L. Beverley, A. W. Boylston, and D. C. Linch. Different pathways of human T cell activation revealed by PHA-P and PHA-M. Immunol. 57:55, (1986).Google Scholar
  5. 5.
    M. J. Weiss, J. F. Daley, J. C. Hodgdon, and E. L. Reinherz. Calcium-dependency of antigen-specific (T3-Ti) and alternative (T11) pathways of human T-cell activation. Proc. Natl. Acad. Sci. USA 81:6836, (1984).ADSCrossRefGoogle Scholar
  6. 6.
    M. E. Kaplan, and C. Clark. An improved rosetting assay for detection of human T lymphocytes. J. Immunol. Meth. 5:131, (1974).CrossRefGoogle Scholar
  7. 7.
    I. Ando, G. Hariri, D. L. Wallace, and P. C. L. Beverley. Tumour promoter phorbol esters induce unresponsiveness to antigen and expression of interleukin 2 receptors on T cells. Eur. J. Immunol. 15:196. (1985).CrossRefGoogle Scholar
  8. 8.
    P. C. L. Beverley, and R. E. Callard. Distinctive functional characteristics of human “T” lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur. J. Immunol. 11:329, (1981).CrossRefGoogle Scholar
  9. 9.
    W. J. M. Tax, H. W. Willems, P. P. M. Reekers, J. A. Capei, and R. A. P. Koene. Polymorphism in mitogenic effects of IgG1 monoclonal antibodies against T3 antigen on human T cells. Nature 304:445, (1983).ADSCrossRefGoogle Scholar
  10. 10.
    P. J. Martin, G. Longton, J. A. Ledbetter, W. Newman, M. P. Braun, P. G. Beatty, and J. A. Hansen. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J. Immunol. 131:180, (1983).Google Scholar
  11. 11.
    E. L. Reinherz, B. F. Haynes, L. M. Nadler, and I. D. Bernstein eds. Leucocyte Typing II, Springer-Verlag, New York in press (1986).Google Scholar
  12. 12.
    N. M. Hogg, S. MacDonald, M. Slusarenko, and P. C. L. Beverley. Monoclonal antibodies specific for human monocytes, granulocytes and endothelium. Immunol. 53:753, (1984).Google Scholar
  13. 13.
    L. L. Lanier, A. M. Le, J. H. Phillips, N. L. Warner, and G. F. Babcock. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J. Immunol. 131:1789, (1983).Google Scholar
  14. 14.
    Y. Bai, P. C. L. Beverley, R. W. Knowles, and W. F. Bodmer. Two monoclonal antibodies identifying a subset of human peripheral blood mononuclear cells with NK and K cell activity. Eur. J. Immunol. 13:521, (1983).CrossRefGoogle Scholar
  15. 15.
    F. M. Brodsky, P. Parham, C. J. Barnstaple, M. J. Crumpton, and W. F. Bodmer. Monoclonal antibodies for analysis of the HLA system. Immunol. Rev. 47:3, (1979).CrossRefGoogle Scholar
  16. 16.
    A. Bernard, L. Bournsell, J. Dansset, C. Milstein, and S. F. Schlossman (eds). Leucocyte Typing I, Springer-Verlag, Berlin (1984).Google Scholar
  17. 17.
    W. J. Leonard, J. M. Depper, T. Uchiyama, K. A. Smith, T. A. Waldmann, and W. C. Greene. A monoclonal antibody that appears to recognize the receptor for human T-cell growth factor: partial characterization of the receptor. Nature 300:2267, (1982).CrossRefGoogle Scholar
  18. 18.
    K. O’Flynn, E. D. Zanders, J. R. Lamb, P. C. L. Beverley, D. L. Wallace, O. E. R. Tatham, W. J. M. Tax, and D. C. Linch. Investigation of the effects of early T-cell activation: analysis of the effects of specific antigen, IL-2 and monoclonal antibodies on intracellular free calcium concentration. Eur. J. Immunol. 15:7, (1985).CrossRefGoogle Scholar
  19. 19.
    P. C. L. Beverley, K. O’Flynn, D. L. Wallace, J. R. Lamb, A. W. Boylston, and D. C. Linch. Regulation of activation and proliferation in T cells. In Leucocyte Typing II, E. L. Reinherz, B. F. Haynes, L. M. Nadler, I. W. Bernstein (eds.) Springer-Verlag New York, (1986) in press.Google Scholar
  20. 20.
    R. Palacios. Mechanisms by which accessory cells contribute in growth of resting T lymphocytes initiated by OKT3 antibody. Eur. J. Immunol. 15:645, (1985).CrossRefGoogle Scholar
  21. 21.
    S. B. Mizel, D. L. Rosenstreich, and J. J. Oppenheim. Phorbol myristate acetate stimulates LAF production by the macrophage cell line P388D1. Cell Immunol. 40:230, (1978).CrossRefGoogle Scholar
  22. 22.
    K. O’Flynn, E. D. Zanders, J. R. Lamb, and P. C. L. Beverley. Phytohaemagglutinin activation of T cells through the sheep red blood cell receptor. Nature 313:686, (1985).CrossRefGoogle Scholar
  23. 23.
    A. C. Meuer, R. E. Hussey, D. A. Cantrell, J. C. Hodgdon, S. F. Schlossman, K. A. Smith, and E. L. Reinherz. Triggering of the T3-Ti antigen-receptor complex results in clonal T-cell proliferation through an interleukin 2-dependent autocrine pathway. Proc. Natl. Acad. Sci. USA 81:1509. ().Google Scholar
  24. 24.
    K. A. Smith, and D. A. Cantrell. Interleukin 2 regulates its own receptors. Proc. Natl. Acad. Sci. USA 82:864, (1985).ADSCrossRefGoogle Scholar
  25. 25.
    Y. Nishizuka. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature 308:693, (1984).ADSCrossRefGoogle Scholar
  26. 26.
    R. Palacios, and D. Martinez-Maza. Is the E receptor on human T-lymphocytes a ‘negative signal receptor’? J. Immunol. 129:2479, (1982).Google Scholar
  27. 27.
    P. J. Martin, G. Longton, J. A. Ledbetter, W. Newman, M. P. Brann, P. A. Beatty, and J. A. Hansen. Identification and functional characterization of two distinct epitopes on the human T-cell surface protein Tp 50. J. Immunol. 131:180, (1983).Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • P. C. L. Beverley
    • 1
  • D. L. Wallace
    • 1
  • K. O’Flynn
    • 2
  • D. C. Linch
    • 2
  1. 1.ICRF Human Tumour Immunology Group, Faculty of Clinical SciencesUniversity CollegeLondonUK
  2. 2.Department of Haematology, Faculty of Clinical SciencesUniversity CollegeLondonUK

Personalised recommendations