The Role of the Antigen Receptor/T3 Complex in T-Cell Activation
The results of several studies demonstrate that activation of resting T-cells requires two stimuli (1–4). One stimulus is represented by the recognition of antigen in conjunction with MHC gene products by the T-cell antigen receptor. The second stimulus is represented by accessory cells and their products. The delineation of the T-cell receptor for antigen and it’s associated molecules, T3, allows a study of how stimuli transmitted through these molecules signal activation. We present here some of these studies. To perform them, we have used the human T-cell line, Jurkat.
KeywordscDNA Probe Antigen Receptor Sodium Butyrate Phorbol Myristate Acetate Inositol Trisphosphate
Unable to display preview. Download preview PDF.
- 1.A. Weiss, R. Wiskocil, and J. Stobo. The role of T3 surface molecules in the activation of human T cells: A two stimulus requirement for IL-2 production reflects events occurring at a pre-translational level. J. Immunol. 133:123, (1984).Google Scholar
- 4.J. Imboden, A. Weiss, and J. Stobo. The antigen receptor on a human T cell line initiates activation by increasing cytoplasmic free calcium. J. Immunol. 134:663, (1985).Google Scholar
- 6.J. Imboden, A. Sloan, A. Weiss, and J. Stobo. The role of Ca2+ mobilization in the T cell antigen receptor-mediated increase in cytoplasmic free calcium. Submitted for publication.Google Scholar