Advertisement

The Role of the Antigen Receptor/T3 Complex in T-Cell Activation

  • Arthur Weiss
  • John Imboden
  • Ken Hardy
  • John Stobo
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 213)

Abstract

The results of several studies demonstrate that activation of resting T-cells requires two stimuli (1–4). One stimulus is represented by the recognition of antigen in conjunction with MHC gene products by the T-cell antigen receptor. The second stimulus is represented by accessory cells and their products. The delineation of the T-cell receptor for antigen and it’s associated molecules, T3, allows a study of how stimuli transmitted through these molecules signal activation. We present here some of these studies. To perform them, we have used the human T-cell line, Jurkat.

Keywords

cDNA Probe Antigen Receptor Sodium Butyrate Phorbol Myristate Acetate Inositol Trisphosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. Weiss, R. Wiskocil, and J. Stobo. The role of T3 surface molecules in the activation of human T cells: A two stimulus requirement for IL-2 production reflects events occurring at a pre-translational level. J. Immunol. 133:123, (1984).Google Scholar
  2. 2.
    A. Weiss, J. Imboden, D. Shoback, and J. Stobo. Role of the T3 surface molecules in human T cell activation: T3 dependent activation results in a rise in cytoplasmic free calcium. Proc. Natl. Acad. Sci. USA 81:4169, (1984).ADSCrossRefGoogle Scholar
  3. 3.
    A. Truneh, F. Albert, P. Goldstein, and A. Schmitt-Verhulst. Early steps of lymphocyte activation bypassed by synergy between calcium ionophores and phorbol ester. Nature 313:318, (1985).ADSCrossRefGoogle Scholar
  4. 4.
    J. Imboden, A. Weiss, and J. Stobo. The antigen receptor on a human T cell line initiates activation by increasing cytoplasmic free calcium. J. Immunol. 134:663, (1985).Google Scholar
  5. 5.
    G. D. Ginder, M. J. Whitters, and J. K. Pohlman. Activation of a chicken embryonic globin gene in adult erythroid cells by 5-azacytidine and sodium butyrate. Proc. Natl. Acad. Sci. USA 81:3954–3958, (1984).ADSCrossRefGoogle Scholar
  6. 6.
    J. Imboden, A. Sloan, A. Weiss, and J. Stobo. The role of Ca2+ mobilization in the T cell antigen receptor-mediated increase in cytoplasmic free calcium. Submitted for publication.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Arthur Weiss
    • 1
  • John Imboden
    • 1
  • Ken Hardy
    • 1
  • John Stobo
    • 1
  1. 1.The Howard Hughes Medical Institute, The Department of MedicineThe University of CaliforniaSan FranciscoUSA

Personalised recommendations