Cell Growth Associated Regulation of c-myc and c-fos in Normal Human T Cells
The mechanism whereby a quiescent lymphocyte is activated to proliferate and express differentiated functions is a fundamental question for understanding immune regulation and growth control. The binding of ligands such as antigen, mitogenic lectins, and some cell surface-specific antibodies to their membrane receptors on lymphocytes generates a transmembrane signal resulting in a variety of metabolic changes culminating after 24 hours in DNA synthesis (1,2). One initial consequence of transmembrane signalling is the expression of mRNA species novel to activated cells. For example, the cellular proto-oncogene c-myc (3), the T cell growth factor, IL-2 (4), and the IL-2 receptor (5,6) increase expression within approximately 1, 12, and 6 hours, respectively, following mitogenic stimulation of T cells. In additional mRNA species, the proto-oncogene c-fos, between 30 and 90 minutes after PHA addition to human T cells. Such increases occur in the presence of protein syrithesis inhibitors (3,6), indicating that mRNA induction is a direct result of the signals that follow receptor binding and as such probably represent primary transcriptional events.
KeywordsCompetence Gene Centrifugal Elutriation Burkitt Lymphoma Cell Representative Cell Volume Mitogenic Lectin
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