Inhibition of Human B Cell Proliferation By Ion Channel Blockers
Resting B cells can be activated by cross-linkage of their membrane immunoglobulins (Ig)(1–4). Anti-Ig antibodies, especially anti-IgM, causes resting B cells to enter and to progress through G1 phase of the cell cycle (5–6). B cells activated with anti-IgM antibody have shown to express Interleukin-2 receptors (IL-2R) and proliferate in response to exogenous IL-2 (7,8). Recently, calcium and calcium-activated K+ channels have been demonstrated in B cell hybridomas and B cell lymphoma lines (9–11). The role of these ion channels in B cell activation, proliferation and differentiation is however, not clearly understood. In this investigation, we have taken an indirect pharmacological approach to study a role of ion channels in anti-IgM-stimulated human B cell activation and proliferation.
KeywordsChannel Blocker Thymidine Incorporation Cyanogen Bromide Peripheral Blood Mononuclear Cell Proliferation Membrane Immunoglobulin
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