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Dissection of the Molecular Events Occurring During T Cell Cycle Progression

  • Kendall A. Smith
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 213)

Abstract

For the first time it has been possible to study the events occurring subsequent to T cell receptor activation with reasonable certainty that the experimental results can be interpreted correctly. We stand at this threshold as a consequence of the identification of the Ti-Tn complex as the receptor for antigen, and the generation of both antagonistic and agonistic monoclonal antibodies reactive with this receptor (1–9). Thus, it has been discerned that activation of T-T3 leads to a rapid (within minutes) increase in intracellular free calcium, and translocation of the calcium-dependent protein kinase (c-kinase) from the cytosol to the membrane (12,13). Subsequently, within a few hours, it is possible to detect transcription of several genes, including those encoding interleukin 2 (IL-2), IL-2 receptors, interferon gamma (IFN-γ) and the c-myc proto-onncogene (14). Moreover, the genes encoding the IL-2 receptor, IFN-γ and c-myc are induced coordinately even in the presence of cycloheximide, thereby suggesting that a simple pre-existing biochemical pathway is responsible for activating these genes (14). However, despite the recognition that activation of both the antigen receptor and the IL-2 receptor are required for T cell proliferation, the precise function of each of these receptors as regards movement of the t cell through the cell cycle has remained obscure. Thus, it has been unclear as to whether activation of the T cell antigen receptor complex promotes movement of the cells through G1 to a point that requires IL-2 just before S-phase, or whether IL-2 itself is responsible for G1 progression.

Keywords

Chicken Embryo Fibroblast Intracellular Free Calcium Cell Antigen Receptor Cell Receptor Activation Cytoplasmic Free Calcium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Kendall A. Smith
    • 1
  1. 1.The Department of MedicineDartmouth Medical SchoolHanoverUSA

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