Inhibition of K-Channels in Insulin Secreting Cells

  • O. H. Petersen
  • I. Findlay
  • M. J. Dunne
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 211)


The major physiological stimulus for insulin secretion from pancreatic β-cells is an elevation of the plasma glucose concentration. The initial effect is to evoke membrane depolarization4 followed by a cyclical pattern of electrical activity consisting of slow waves of depolarization with action potential-like spikes.9,12 Tracer flux studies have indicated that glucose metabolism is associated with a decrease in membrane K+ permeability17 and Ashcroft, Harrison and Ashcroft2 have demonstrated glucose-induced closure of single K-channels in isolated rat pancreatic β-cells (this book).


Pancreatic Islet Cell Membrane Patch Glyceraldehyde Phosphate Excise Membrane Patches3 Plasma Glucose Concentra 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    F. M. Ashcroft, S. J. H. Ashcroft, and D. E. Harrison, The glucose-sensitive potassium channel in rat pancreatic beta-cells is inhibited by intracellular ATP, J. Physiol. (Lond.) 369:101P (1985).Google Scholar
  2. 2.
    F. M. Ashcroft, D. E. Harrison, and S. J. Ashcroft, Glucose induces closure of single potassium channels in isolated rat pancreatic beta-cells, Nature 312:446 (1984).PubMedCrossRefGoogle Scholar
  3. 3.
    D. L. Cook and C. N. Hales, Intracellular ATP directly blocks K+ channels in pancreatic β-cells, Nature 311:271 (1984).PubMedCrossRefGoogle Scholar
  4. 4.
    P. M. Dean and E. K. Matthews, Electrical activity in pancreatic islet cells, Nature 219:389 (1968).PubMedCrossRefGoogle Scholar
  5. 5.
    P. M. Dean, E. K. Matthews, and Y. Sakamoto, Pancreatic islet cells: effects of mono-saccharides, glycolytic intermediates and metabolic inhibitors on membrane potential and electrical activity. J. Physiol. 246:459 (1985).Google Scholar
  6. 6.
    I. Findlay and M. J. Dunne, Voltage-activated Ca2+ currents in insulin-secreting cells, FEBS Lett. 189:281 (1985).PubMedCrossRefGoogle Scholar
  7. 7.
    I. Findlay, M. J. Dunne, and O. H. Petersen, ATP-sensitive inward rectifier and voltage- and calcium-activated K+ channels in cultured pancreatic islet cells, J. Membr. Biol. 88:165 (1985).PubMedCrossRefGoogle Scholar
  8. 8.
    I. Findlay, M. J. Dunne, S. Ullrich, C. B. Wollheim, and O. H. Petersen, Quinine inhibits independent K+ channels whereas tetraethylammonium inhibits Ca2+-activated K+ channels in insulin-secreting cells, FEBS Lett. 185:4 (1985).PubMedCrossRefGoogle Scholar
  9. 9.
    J. C. Henquin and H. P. Meissner, Significance of ionic fluxes and changes in membrane potential for stimulus-secretion coupling in pancreatic β-cells, Experientia, 40:1043 (1984).PubMedCrossRefGoogle Scholar
  10. 10.
    M. Kakei, A. Noma, and T. Shibasaki, Properties of adenosine-triphosphate-regulated potassium channels in quinea-pig ventricular cells, J. Physiol. (Lond.) 363:441 (1985).Google Scholar
  11. 11.
    W. J. Malaisse and A. Herchuelz, Nutritional regulation of K+ conductance: an unsettled aspect of pancreatic β cell physiology, in: “Biochemical Actions of Hormones, Vol. IX,” G. Litwack, ed., Academic Press, N.Y., pp. 69–92 (1982).CrossRefGoogle Scholar
  12. 12.
    O. H. Petersen, “Electrophysiology of gland cells,” Academic Press, London (1980).Google Scholar
  13. 13.
    G. A. Praz, P. A. Halban, C. B. Wollheim, B. Blondel, A. J. Strauss, and A. E. Renold, Regulation of immunoreactive-insulin release from a rat cell line (RINm5F), Biochem. J. 210:345 (1983).PubMedGoogle Scholar
  14. 14.
    P. Rorsman and G. Trube, Glucose-dependent K+ channels in pancreatic β-cells are regulated by intracellular ATP, Pflugers Arch. 405:305 (1985).PubMedCrossRefGoogle Scholar
  15. 15.
    P. Rorsman and G. Trube, Calcium and delayed potassium currents in mouse pancreatic β-cells under voltage-clamp conditions, J. Physiol. (Lond.), (in press 1986).Google Scholar
  16. 16.
    L. S. Satin and D. L. Cook, Voltage-gated Ca2+ current in pancreatic β-cells, Pflugers Arch. 404:385 (1985).PubMedCrossRefGoogle Scholar
  17. 17.
    J. Sehlin and I.-B. Taljedal, Glucose-induced decrease in Rb+ permeability in pancreatic β-cells, Nature 253:635 (1975).PubMedCrossRefGoogle Scholar
  18. 18.
    C. B. Wollheim and G. W. G. Sharp, Regulation of insulin release by calcium, Physiol. Rev. 61: 914 (1981).PubMedGoogle Scholar
  19. 19.
    C. B. Wollheim, S. Ullrich, and T. Pozzan, Glyceraldehyde, but not cyclic AMP-stimulated insulin release is preceded by a rise in cytosolic free Ca2+, FEBS Lett. 177:17 (1984).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • O. H. Petersen
    • 1
  • I. Findlay
    • 1
  • M. J. Dunne
    • 1
  1. 1.M. R. C. Secretory Control Research Group, The Physiological LaboratoryUniversity of LiverpoolLiverpoolUK

Personalised recommendations