K-Channels in an Insulin-Secreting Cell Line: Effects of ATP and Sulphonylureas

  • M. L. J. Ashford
  • N. C. Sturgess
  • D. L. Cook
  • C. N. Hales
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 211)

Abstract

The recent application of the patch-clamp technique to the study of single channels present in the β-cell plasma membrane has led to the discovery of a channel the activity of which is inhibited by the application of ATP to the membrane cytoplasmic surface7. It has been suggested that this channel may be responsible for glucose-induced β-cell depolarisation and that ATP may be the long-sought connection between glucose metabolism and the excitation of insulin secretion7,8. Cell lines are proving to be very convenient and reproducible systems with which to study metabolic processes and their regulation and we have found them particularly suitable for patch clamp procedures. We have therefore investigated a new insulin-secreting cell line5 using the patch-clamp technique in order to study potassium channels and their role in the control of insulin secretion.

Keywords

Permeability Adenosine MgCl Polypeptide Cyanide 

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • M. L. J. Ashford
    • 1
  • N. C. Sturgess
    • 1
    • 2
  • D. L. Cook
    • 3
  • C. N. Hales
    • 2
  1. 1.Department of PharmacologyUniversity of CambridgeCambridgeEngland
  2. 2.Department of Clinical BiochemistryUniversity of CambridgeCambridgeEngland
  3. 3.Departments of Physiology and Biophysics, and MedicineUniversity of Washington and Seattle VA Medical CenterSeattleUSA

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