K-Channels in an Insulin-Secreting Cell Line: Effects of ATP and Sulphonylureas

  • M. L. J. Ashford
  • N. C. Sturgess
  • D. L. Cook
  • C. N. Hales
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 211)


The recent application of the patch-clamp technique to the study of single channels present in the β-cell plasma membrane has led to the discovery of a channel the activity of which is inhibited by the application of ATP to the membrane cytoplasmic surface7. It has been suggested that this channel may be responsible for glucose-induced β-cell depolarisation and that ATP may be the long-sought connection between glucose metabolism and the excitation of insulin secretion7,8. Cell lines are proving to be very convenient and reproducible systems with which to study metabolic processes and their regulation and we have found them particularly suitable for patch clamp procedures. We have therefore investigated a new insulin-secreting cell line5 using the patch-clamp technique in order to study potassium channels and their role in the control of insulin secretion.


Potassium Permeability Single Channel Opening Ischaemic Contracture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    F.M. Ashcroft, D.E. Harrison, and S.J.H. Ashcroft, Glucose induces closure of single potassium channels in isolated rat pancreatic cells, Nature 312;446 (1984).PubMedCrossRefGoogle Scholar
  2. 2.
    S.J.H. Ashcroft, L.C.C Weerasinghe, and P.J. Randle, Interrelationship of islet metabolism, adenosine triphosphate content and insulin release, Biochem. J 132:223 (1973).PubMedGoogle Scholar
  3. 3.
    I. Atwater, B. Ribalet, and E. Rojas, Cyclic changes in potential and resistance of the β-cell membrane induced by glucose in islets of Langerhans from mouse, J. Physiol. 278:117 (1978).PubMedGoogle Scholar
  4. 4.
    O.L. Bricknell, P.S. Daries, and L.H. Opie, A relationship between adenosine triphosphate, glycolysis and ischaemic contracture in the isolated rat heart, J. Molec. and Cell Cardiol. 13:941 (1981).CrossRefGoogle Scholar
  5. 5.
    C.A. Carrington, E.D. Rubery, E.C. Pearson, and C.N. Hales, Five new insulin-producing cell lines with differing secretory properties, J. Endocr. 109:in press(1986).Google Scholar
  6. 6.
    W.L. Chick, S. Warren, R.N. Chute, A.A. Like, V. Lauris and K.C. Kitchen, A transplantable insulinoma in the rat, Proc. Natl. Acad. Sci. USA 74:628 (1977).PubMedCrossRefGoogle Scholar
  7. 7.
    D.L. Cook and C.N. Hales, Intracellular ATP directly blocks K+ channels in pancreatic β-cells, Nature 311:271 (1984).PubMedCrossRefGoogle Scholar
  8. 8.
    D.L. Cook, C.N. Hales, and L.S. Satin, Glucose suppresses ATP-inhibited K-channels in pancreatic β-cells. This volume (1986).Google Scholar
  9. 9.
    O.P. Hamill, A. Marty, E. Neher, B. Sakmann and F.J. Sigworth, Improved patch-clamp techniques for high resolution current recording from cells and cell-free membrane patches, Pflügers Arch. 391:85 (1981).PubMedCrossRefGoogle Scholar
  10. 10.
    J.-C. Henquin, Tolbutamide stimulation and inhibition of insulinrelease: studies of the underlying ionic mechanisms in isolated rat islets, Diabetologia 18:151 (1980).PubMedCrossRefGoogle Scholar
  11. 11.
    T.J.C. Higgins and P.J. Bailey, The effects of cyanide and iodoacetate intoxication and ischaemia on enzyme release from the perfused rat heart, Biochim. Biophys. Acta. 762:67 (1983).PubMedCrossRefGoogle Scholar
  12. 12.
    H.E. Lebovitz, Oral hypoglycaemic agents, in: “The Diabetes Annual”, K.G.M.M. Alberti and L.P. Krall, eds., Elsevier, 1:93 (1985).Google Scholar
  13. 13.
    W.J. Malaisse, J.C. Hutton, S. Kawazu, A. Herchuelz, I. Valverde, and A. Sener, The stimulus-secretion coupling of glucose-induced insulin release. XXXV, The links between metabolic and cationic events, Diabetologia 16:331 (1979).PubMedCrossRefGoogle Scholar
  14. 14.
    A. Noma, ATP-regulated K+ channels in cardiac muscle, Nature 305:147 (1983).PubMedCrossRefGoogle Scholar
  15. 15.
    N.C. Sturgess, M.L.J. Ashford, C.A. Carrington, and C.N. Hales Single Channel Recordings of Potassium Currents in an Insulin Secreting Cell Line, J. Endocr. 109: in press (1986).Google Scholar
  16. 16.
    N.C. Sturgess, M.L.J. Ashford, D.L. Cook, and C.N. Hales, The sulphonylurea receptor may be an ATP-sensitive potassium channel, Lancet ii:474 (1985).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • M. L. J. Ashford
    • 1
  • N. C. Sturgess
    • 1
    • 2
  • D. L. Cook
    • 3
  • C. N. Hales
    • 2
  1. 1.Department of PharmacologyUniversity of CambridgeCambridgeEngland
  2. 2.Department of Clinical BiochemistryUniversity of CambridgeCambridgeEngland
  3. 3.Departments of Physiology and Biophysics, and MedicineUniversity of Washington and Seattle VA Medical CenterSeattleUSA

Personalised recommendations