Pulsatile Insulin Release and Electrical Activity from Single ob/ob Mouse Islets of Langerhans
Since Ca-action potentials were first measured in β-cells, it has been assumed that the resultant Ca2+ influx provides a critical Ca2+-dependent step in the events leading to insulin secretion.4,6. The correlation between electrical activity and insulin release remained indirect, however, since membrane potentials were recorded from single β-cells within an isolated islet and insulin release was measured from batches of collagenase isolated islets (usually more than 100) or from the perfused pancreas (containing over 1000 islets).
KeywordsMembrane Potential Electrical Activity Insulin Release High Glucose Concentration Mouse Islet
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