Pulsatile Insulin Release and Electrical Activity from Single ob/ob Mouse Islets of Langerhans

  • L. M. Rosario
  • I. Atwater
  • A. M. Scott
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 211)


Since Ca-action potentials were first measured in β-cells, it has been assumed that the resultant Ca2+ influx provides a critical Ca2+-dependent step in the events leading to insulin secretion.4,6. The correlation between electrical activity and insulin release remained indirect, however, since membrane potentials were recorded from single β-cells within an isolated islet and insulin release was measured from batches of collagenase isolated islets (usually more than 100) or from the perfused pancreas (containing over 1000 islets).


Membrane Potential Electrical Activity Insulin Release High Glucose Concentration Mouse Islet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    I. Atwater, B. J. Frankel, E. Rojas, and G. M. Grodsky, β-cell membrane potential and insulin release; role of calcium and calcium:magnesium ratio, Quart. J. Exp. Physiol. 68:233 (1983).Google Scholar
  2. 2.
    I. Atwater, E. Rojas, and A. Scott, Simultaneous measurements of insulin release and electrical activity from single micro-dissected mouse islets of Langerhans, J. Physiol. 291:57P (1979).PubMedGoogle Scholar
  3. 3.
    P. M. Beigelman, L. J. Thomas, B. Slavin, M. J. Shu, and S. P. Bessman, Insulin from individual isolated islets of Langerhans. I: response to glucose, Biochem. Med. 8:392 (1973).Google Scholar
  4. 4.
    C. M. Dawson, I. Atwater, and E. Rojas, Potassium-induced insulin release and voltage noise measurements in single mouse islets of Langerhans, J. Membrane Biol. 64:33 (1982).CrossRefGoogle Scholar
  5. 5.
    G. T. Eddlestone, A. Goncalves, J. A. Bangham, and E. Rojas, Electrical coupling between cells in islets of Langerhans from mouse, J. Membrane Biol. 77:1 (1984).CrossRefGoogle Scholar
  6. 6.
    J. C. Henquin and H. P. Meissner, Significance of ionic fluxes and changes in membrane potential for stimulus-secretion coupling in pancreatic $-cells, Experientia 40:1043 (1984).PubMedCrossRefGoogle Scholar
  7. 7.
    P. Meda, I. Atwater, A. Goncalves, A. Bangham, L. Orci, and E. Rojas, The topography of electrical synchrony among β-cells in the mouse islet of Langerhans, Quart. J. Exp. Physiol. 69:719 (1984).Google Scholar
  8. 8.
    H. P. Meissner and M. Preissler, Ionic mechanisms of the glucose-induced membrane potential changes in β-cells, Horm. Metab. Res. (Suppl.) 10:100 (1980).Google Scholar
  9. 9.
    E. M. Perez-Armendariz and I. Atwater, Glucose-evoked changes in the extracellular concentration of potassium and calcium in mouse islets of Langerhans, this book.Google Scholar
  10. 10.
    E. Perez-Armendariz, I. Atwater, and E. Rojas, Glucose-induced oscillatory changes in extracellular ionized potassium concentration in mouse islets of Langerhans, Biophys. J. 48:741 (1985).PubMedCrossRefGoogle Scholar
  11. 11.
    L. M. Rosario, I. Atwater, and E. Rojas, Membrane potential measurements in islets of Langerhans from ob/ob obese mice suggest an alteration in [Ca2+]i-activated K+ permeability, Quart. J. Exp. Physiol. 70:137 (1985).Google Scholar
  12. 12.
    A. M. Scott, I. Atwater, and E. Rojas, A method for the simultaneous measurement of insulin release and β-cell membrane potential in single mouse islets of Langerhans, Diabetologia 21:470 (1981).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • L. M. Rosario
    • 1
  • I. Atwater
    • 1
  • A. M. Scott
    • 2
  1. 1.Laboratory of Cell Biology and Genetics, NIDDKNational Institutes of HealthBethesdaUSA
  2. 2.Department of Biophysics, School of Biological SciencesUniversity of East AngliaNorwichEngland

Personalised recommendations