Advertisement

Effects of Monensin on Glucose-Induced Insulin Release and 45Ca2+ Outflow

  • A. Kanatsuka
  • H. Makino
  • N. Hashimoto
  • M. Sakurada
  • S. Yoshida
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 211)

Abstract

The β-cells in the pancreatic islets respond to glucose with a biphasic insulin release2. Protons generated from glucose metabolism have been proposed to be a coupling factor between metabolic and ionic events in the cells1,7. A rise in cytosol Ca2+ concentration is considered to be essential in stimulus-secretion coupling in the β-cells4. Thus, it seems possible that the biphasic insulin release is a reflection of a biphasic change in the Ca2+ concentration of the cytosol, the change being controlled by the rate of generation of protons. To examine the role of protons and calcium in the insulin response to glucose, we examined the effect of monensin, a carboxylic ionophore8, on the first and second phase of glucose-induced insulin release and on the 45Ca2+ efflux from perifused rat pancreatic islets.

Keywords

Insulin Release Biphasic Change Perifusion System Inhibit Insulin Release Fractional Outflow Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A.R. Carpinelli, A. Sener, A. Herchuelz, and W.J. Malaisse, Stimulussecretion coupling of glucose-induced insulin release. Effect of intracellular acidification upon calcium efflux from islet cells, Metabolism 29:540 (1980).PubMedCrossRefGoogle Scholar
  2. 2.
    Ü.L. Curry, L.L. Bennet, and G.M. Grodsky, Dynamics of insulin secretion by the perfused rat pancreas, Endocrinology 83:572 (1968).PubMedCrossRefGoogle Scholar
  3. 3.
    G. Gold, J. Pou, R.M. Nowlain, and G.M. Grodsky, Effect of monensin on conversion of proinsulin and secretion of newly synthesized insulin in isolated rat islets, Diabetes 33:1019 (1984).PubMedCrossRefGoogle Scholar
  4. 4.
    G.M. Grodsky and L.L. Bennet, Cation requirements for insulin secretion in the isolated perfused pancreas, Diabetes 15:910 (1966).PubMedGoogle Scholar
  5. 5.
    A. Kanatsuka, H. Makino, M. Osegawa, J. Kasanuki, T. Suzuki, and S. Yoshida, Is somatostatin a true local inhibitory regulator of insulin secretion, Diabetes 33:510 (1984).PubMedCrossRefGoogle Scholar
  6. 6.
    W.J. Malaisse, A.R. Carpinelli, and A. Sener, Stimulus-secretion coupling of glucose-induced insulin release. Timing of early metabolic, ionic, and secretory events, Metabolism 30:527 (1981).PubMedCrossRefGoogle Scholar
  7. 7.
    C.S. Pace, Role of pH as a transduction device in triggering electrical and secretory responses in islets β-cells, Fed. Proc. 43:2379 (1984).PubMedGoogle Scholar
  8. 8.
    B.C. Pressman, Biological applications of ionophores, Annu. Rev. Biochem. 45:501 (1976).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • A. Kanatsuka
    • 1
  • H. Makino
    • 1
  • N. Hashimoto
    • 1
  • M. Sakurada
    • 1
  • S. Yoshida
    • 1
  1. 1.The Second Department of Internal MedicineChiba University School of MedicineChiba 280Japan

Personalised recommendations