Effects of Monensin on Glucose-Induced Insulin Release and 45Ca2+ Outflow
Part of the
Advances in Experimental Medicine and Biology
book series (AEMB, volume 211)
The β-cells in the pancreatic islets respond to glucose with a biphasic insulin release2. Protons generated from glucose metabolism have been proposed to be a coupling factor between metabolic and ionic events in the cells1,7. A rise in cytosol Ca2+ concentration is considered to be essential in stimulus-secretion coupling in the β-cells4. Thus, it seems possible that the biphasic insulin release is a reflection of a biphasic change in the Ca2+ concentration of the cytosol, the change being controlled by the rate of generation of protons. To examine the role of protons and calcium in the insulin response to glucose, we examined the effect of monensin, a carboxylic ionophore8, on the first and second phase of glucose-induced insulin release and on the 45Ca2+ efflux from perifused rat pancreatic islets.
KeywordsInsulin Release Biphasic Change Perifusion System Inhibit Insulin Release Fractional Outflow Rate
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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