Electrophysiological Evidence for Histaminergic Modulation of Pancreatic β-Cell Function
Although it is known that amines affect insulin secretion, their effects on glucose-induced insulin secretion are difficult to interpret for several reasons: 1) differential effects of alpha- and beta-receptor stimulation by catecholamines, 2) induction of catecholamine secretion by serotonin, 3) changes of capillary blood flow by histamine or 4) degranula-tion of β-cells with a marked dilatation of the rough endoplasmic reticulum, induced by the histamine and serotonin antagonist cyproheptadine. Membrane potential recording provides a direct assay for the effects of these substances on pancreatic β-cell membrane properties. This technique has been successfully used to further understand the effects of catecholamines on the β-cell membrane7. The aim of the present study was to determine the role of the putative neurotransmitter, histamine, on the control of membrane potential in the mouse pancreatic β-cell.
KeywordsCapillary Blood Flow Transient Inhibition Culture Dorsal Root Ganglion Membrane Potential Recording Inhibitory Synaptic Potential
Unable to display preview. Download preview PDF.
- 4.R. Ferrer, S. Sala, J.V. Sanchez-Andres, and B. Soria, Further evidence that Zn blocks voltage-dependent Ca channels in the mouse pancreatic 3-cell, Biochem. Soc. Trans. 13:680 (1985).Google Scholar
- 7.S. de Sa Santana, R. Ferrer, E. Rojas, and I. Atwater, Effects of adrenaline and noradrenaline on glucose-induced electrical activity of mouse pancreatic 3-cell, Quart. J. exp. Physiol. 68:247 (1983).Google Scholar
- 8.B.S.R. Sastry and J.W. Phyllis, Evidence for an ascending inhibitory histaminergic pathway to the cerebral cortex, J. Physiol. 54:782 (1976).Google Scholar
- 9.B. Soria, R. Ferrer, I. Atwater, and E. Rojas, Graded electrogenesis in pancreatic β-cell, in: “Biophysics of the Pancreatic β-cell”, Eds: I. Atwater, E. Rojas and B. Soria, Plenum Press, N.Y. this volume. (1986).Google Scholar