Insulin Secretion Studied in Islets Permeabilised by High Voltage Discharge

  • P. M. Jones
  • S. L. Howell
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 211)


Studies of the cellular mechanisms of insulin secretion from intact β-cells are hampered, to some extent, by the existence of an intact plasma membrane which limits the degree to which the intracellular environment can be experimentally manipulated. One means of circumventing this problem is to permeabilise the plasma membrane of the β-cells by a high voltage discharge technique.


Insulin Secretion Insulin Release Phorbol Ester Secretory Response High Voltage Discharge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    L. Best, M. Dunlop, and W.J. Malaisse, Phospholipid metabolism in pancreatic islets, Experientia 40:1085 (1984).PubMedCrossRefGoogle Scholar
  2. 2.
    T.J. Biden, M. Prentki, R.F. Irvine, M.J. Berridge, and C.B. Wollheim, Inositol 1,4,5-trisphosphate mobilizes intracellular Ca2+ from permeabilised insulin-secreting cells, Biochem. J. 223:467 (1984).PubMedGoogle Scholar
  3. 3.
    K.W. Brocklehurst and H.B. Pollard, Enhancement of Ca2+-induced catecholamine release by the phorbol ester TPA in digitonin-permeabilised cultured bovine adrenal chromaffin cells, FEBS Lett. 183:107 (1985).PubMedCrossRefGoogle Scholar
  4. 4.
    M. Castanaga, Y. Takai, K. Kaibuchi, K. Sano, A. Kikkawa, and Y. Nishizuka, Direct activation of calcium-activated phospholipid-dependent protein kinase by tumor-promoting phorbol esters, J. Biol. Chem. 257:7847 (1982).Google Scholar
  5. 5.
    J.R. Colca, B.A. Wolf, P.G. Comens, and M.L. McDaniel, Protein phosphorylation in permeabilised pancreatic islet cells, Biochem. J. 228:529 (1985).PubMedGoogle Scholar
  6. 6.
    S.L. Howell and K.W. Taylor, K ions and the secretion of insulin by islets of Langerhans incubated in vitro, Biochem. J. 108:17 (1968).PubMedGoogle Scholar
  7. 7.
    J.C. Hutton, M. Peshavaria, and K.W. Brocklehurst, Phorbol ester stimulation of insulin release and secretory granule protein phosphorylation in a transplantable rat insulinoma, Biochem. J. 224:483 (1984).PubMedGoogle Scholar
  8. 8.
    D.E. Knight and P.F. Baker, Calcium dependence of catecholamine release from bovine adrenal medullary cells after exposure to intense electric fields, J. Membr. Biol. 68:107 (1982).PubMedCrossRefGoogle Scholar
  9. 9.
    D.E. Knight and P.F. Baker, The phorbol ester TPA increases the affinity of exocytosis for calcium in leaky adrenal medullary cells, FEBS Lett. 160:98 (1983).PubMedCrossRefGoogle Scholar
  10. 10.
    D.E. Knight and E. Koh, Ca and cyclic nucleotide dependence of amylase release from isolated rat pancreatic acinar cells rendered permeable by intense electric field, Cell Calcium 5:401 (1984).PubMedCrossRefGoogle Scholar
  11. 11.
    D.E. Knight and M.C. Scrutton, Cyclic nucleotides control a system which regulates Ca2+ sensitivity of platelet secretion, Nature 309:66 (1984).PubMedCrossRefGoogle Scholar
  12. 12.
    F. Lanter, R.A. Steiner, D. Ammann, and W. Simon, Critical evaluation of the applicability of neutral carrier Ca2+-selective microelectrodes, Anal. Chim. Acta 135:51 (1982).CrossRefGoogle Scholar
  13. 13.
    W.J. Malaisse, P. Lebrun, A. Herchuelz, A. Sener, and A. Malaisse-Lagae, Synergistic effect of a tumor-promoting phorbol ester and a hypoglycemic sulfonylurea upon insulin release, Endocrinology 113:1870 (1983).PubMedCrossRefGoogle Scholar
  14. 14.
    W. Montague, N.G. Morgan, G.M. Rumford, and C.A. Prince, Effect of glucose on polyphosphoinositide metabolism in isolated rat islets of Langerhans, Biochem. J. 227:483 (1985).PubMedGoogle Scholar
  15. 15.
    C.E. Pace and K.T. Goldsmith, Action of a phorbol ester on β-cells: potentiation of stimulant-induced electrical activity, Am. J. Physiol. 248:C527 (1985).PubMedGoogle Scholar
  16. 16.
    C.E. Pace, J.T. Tarvin, A.S. Neighbors, J.A. Pirkle, and M.H. Greider, Use of a high voltage technique to determine the molecular requirements for exocytosis in islet cells, Diabetes 29:911 (1980).PubMedGoogle Scholar
  17. 17.
    H. Portzehl, P.C. Caldwell, and J.C. Ruegg, The dependence of contraction and relaxation of muscle fibres from the crab Maia squindo on the internal concentration of free calcium ions, Biochim. Biophys Acta 70:581 (1964).Google Scholar
  18. 18.
    P. Rorsman, H. Abrahamsson, E. Gylfe, and B. Hellman, Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic β-cells, FEBS Lett. 170:196 (1984).PubMedCrossRefGoogle Scholar
  19. 19.
    J. Stutchfield, F. Sullivan, K.C. Pedley, and S.L. Howell, Effects of polymyxin B, a protein kinase C inhibitor, on insulin release, Diabetes Res. Clin. Practice Supp. 1:S539 (1985).Google Scholar
  20. 20.
    T. Tamagawa, H. Niki, and A. Niki, Insulin release independent of a rise of cytosolic free Ca2+ by forskolin and phorbol ester, FEBS Lett. 183:430 (1985).PubMedCrossRefGoogle Scholar
  21. 21.
    K. Tanigawa, H. Kuzuya, H. Imura, H. Taniguchi, S. Baba, Y. Takai, and Y. Nishizuka, Calcium-activated phospholipid-dependent protein kinase in rat pancreas islets of Langerhans, FEBS Lett. 138:183 (1982).PubMedCrossRefGoogle Scholar
  22. 22.
    M.A.G. Virji, M.W. Steffes, and R.D. Estensen, Phorbol myristate acetate: effects of a tumor promoter on insulin release from isolated rat islets of Langerhans, Endocrinology 102:706 (1978).PubMedCrossRefGoogle Scholar
  23. 23.
    C.B. Wollheim and T. Pozzan, Correlation between cytosolic free Ca2+ and insulin release in an insulin-secreting cell line, J. Biol. Chem. 259:2262 (1984).PubMedGoogle Scholar
  24. 24.
    C.B. Wollheim and G.W.G. Sharp, Regulation of insulin release by calcium, Physiol. Rev. 61:914 (1981).PubMedGoogle Scholar
  25. 25.
    B.A. Wolf, P.G. Comens, K.E. Ackerman, W.R. Sherman, and M.L. McDaniel, The digitonin-permeabilised pancreatic islet model. Biochem. J. 227:965 (1985).PubMedGoogle Scholar
  26. 26.
    S. Yamamoto, T. Nakadate, T. Nakaki, K. Ishii, and R. Kato, Tumor promoter 12-o-tetradecanoylphorbol-13-acetate induced insulin secretion, Biochem. Biophys. Res. Comm1 105:759 (1982).PubMedCrossRefGoogle Scholar
  27. 27.
    M.A. Yaseen, K.C. Pedley, and S.L. Howell, Regulation of insulin secretion from islets of Langerhans rendered permeable by electric discharge, Biochem. J. 206:81 (1982).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • P. M. Jones
    • 1
  • S. L. Howell
    • 1
  1. 1.Department of Physiology, King’s College London (KQC)University of LondonKensington, LondonEngland

Personalised recommendations