Optical Detection of Calcium Dependent ATP Release from Stimulated Medullary Chromaffin Cells

  • E. Rojas
  • E. Forsberg
  • H. B. Pollard
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 211)


It is well known that different types of secretory granules contain adenosine-5′-triphosphate (ATP) in addition to the specific hormones and neurotransmitters. Examples include secretory granules from chromaffin cells,32 pancreatic B-cells,27 platelets4,5,30 and mast cells. We have recently shown22 that it is possible to obtain real-time measurements of the kinetics of secretion of vesicle contents by monitoring ATP release from stimulated medullary chromaffin cells using luciferin-luciferase.


Muscarinic Receptor Secretory Granule Chromaffin Cell Inositol Phosphate Krebs Solution 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • E. Rojas
    • 1
  • E. Forsberg
    • 1
  • H. B. Pollard
    • 1
  1. 1.Laboratory of Cell Biology and Genetics, NIDDKNational Institutes of HealthBethesdaUSA

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