Insulin Release and K+-Induced Depolarization in Mouse Pancreatic β-Cells

  • S. Joost
  • I. Atwater
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 211)


Ca influx through the β-cell membrane represents an important step in the triggering of glucose-induced insulin secretion. The way in which β-cells recognize the glucose concentration and subsequently regulate Ca2+ influx has been the focal point of much of the research in islet physiology over the last two decades. We have studied Ca-channel activity in the β-cell using high external potassium [K+]0 to depolarize the membrane.1 The analysis of the resulting membrane noise revealed that the conductance of a single channel was about 6 pS with a mean open time of about 20 msec at 0 mV.1 Thus, β-cell Ca-channels behave similarly to Ca-channels described in other cells.6


Insulin Release Patch Clamp Mouse Islet Insulin Output Pork Insulin 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • S. Joost
    • 1
  • I. Atwater
    • 1
  1. 1.Laboratory of Cell Biology and Genetics, NIADDKNational Institutes of HealthBethesdaUSA

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