Patch Pipettes Used for Loading Small Cells with Fluorescent Indicator Dyes

  • Erwin Neher
  • Wolf Almers
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 211)


In modern cell physiology, much important information has come from the intracellular application of membrane-impermeant substances, such as ion indicator dyes, second messengers, regulatory proteins, antibodies and messenger RNA. Such substances have been either injected into cells through microelectrodes or loaded into lipid vesicles and erythrocyte ghosts that were later allowed to fuse with the cell membrane. We have monitored cytoplasmic Ca2+ in mast cells with intracellularly applied fura-2, a new Ca-indicator dye.


Mast Cell Series Resistance Patch Pipette Peritoneal Mast Cell Erythrocyte Ghost 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Erwin Neher
    • 1
  • Wolf Almers
    • 2
  1. 1.Max-Planck-Institut fur biophysikalische ChemieGottingenGermany
  2. 2.Dept. of Physiology and Biophysics, SJ-40University of WashingtonSeattleUSA

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