EPR Measurements of Conformational Changes on the E1 Component of the Pyruvate Dehydrogenase Complex From Escherichia Coli

  • Klaus Graupe
  • Hans Bisswanger
Conference paper
Part of the NATO ASI Series book series (NSSA, volume 127)


One of the most important enzymes of cell metabolism is the PDC2, which acts as a link between carbohydrate catabolism and, via the tricarboxylic acid cycle, the energy production. Due to this central role, the enzyme is subject to a great number of regulatory influences. Pyruvate and its precursors activate the enzyme complex, while acetyl CoA and metabolites derived from this product, such as tricarboxylic acid cycle intermediates and the high-energy compounds GTP and ATP, are inhibitors (Schwartz et al., 1968; Shen and Atkinson, 1970; Bisswanger, 1972; Bisswanger and Henning, 1971).


Thiol Group Tricarboxylic Acid Cycle Carbohydrate Catabolism Tricarboxylic Acid Cycle Intermediate Essential Thiol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



pyruvate dehydrogenase complex with the three enzyme components


pyruvate dehydrogenase (EC


dihydrolipoamide acetyltransferase (EC


dihydrolipoamide dehydrogenase ( EC


thiamine diphosphate


N-4'-(2',2',6',6'-tetramethylpiperidino-1'-oxyl)-p-chloromercuribenzoic acid


N-4'-(2',2',6',6'- tetramethylpiperidino-1'-oxyl)-maleimide


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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Klaus Graupe
    • 1
  • Hans Bisswanger
    • 1
  1. 1.Plysiologisch-chemisches InstitutUniversität TübingenTübingenDeutschland

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