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ALS Cerebrospinal Fluid Enhances Human Foetal Astroglial Cell Proliferation in Vitro

  • V. Silani
  • A. Pizzuti
  • L. M. Redaelli
  • R. Bassani
  • I. R. Causarano
  • M. Buscaglia
  • G. Zuliani
  • G. Scarlato
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 209)

Abstract

Studies on the cerebrospinal fluid (CSF) of patients with amyotrophic lateral sclerosis (ALS) are relatively few and somewhat unclear. Recently, we were unable to demonstrate a cytotoxic effect of ALS sera on human central nervous system (CNS) cultures from 15 - 20 week old human foetuses, confirming the negative therapeutic effects obtained using plasma-exchange in affected patients[l]. We decided to study the effects of ALS-CSF on human foetal motor cortex in vitro. This tissue can grow in vitro in the presence of a standard tissue culture medium which contains 10% normal CSF and provides a sensitive assay for trophic factors and toxic substances of clinical relevance. ALS-CSF induces a significant glial cell proliferation in vitro. Indirect immunofluorescence techniques were used to identify the atroglial proliferating cells.

Keywords

Amyotrophic Lateral Sclerosis Motor Cortex Amyotrophic Lateral Sclerosis Patient Motor Neuron Disease Sporadic Amyotrophic Lateral Sclerosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    V. Silani, G. Scarlato, G. Valli, and M. Marconi, Plasma-exchange ineffective in amyotrophic lateral sclerosis, Arc.Neurol., 37: 511–513 (1980).CrossRefGoogle Scholar
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    V. Silani, M. Buscaglia, and G. Scarlato, Neuroimmunology of human foetal brain in tissue culture, Int.J.Dev.Neurosci., Abs. 90 (1984).Google Scholar
  3. 3.
    H. Donnenfeld, R. J. Kascsak, and H. Bartfeld, Deposits of IgG and C3 in the spinal cord and motor cortex of ALS patients, J.Neuroimmunol., 6: 51–57 (1984).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • V. Silani
    • 1
  • A. Pizzuti
    • 1
  • L. M. Redaelli
    • 1
  • R. Bassani
    • 1
  • I. R. Causarano
    • 1
  • M. Buscaglia
    • 2
  • G. Zuliani
    • 2
  • G. Scarlato
    • 2
  1. 1.“Dino Ferrari”, Neuromuscular Disease CenterThe Institute of NeurologyItaly
  2. 2.Obstetrics-Gynecology ClinicUniversity of Milan Medical SchoolMilanItaly

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