ALS Cerebrospinal Fluid Enhances Human Foetal Astroglial Cell Proliferation in Vitro
Studies on the cerebrospinal fluid (CSF) of patients with amyotrophic lateral sclerosis (ALS) are relatively few and somewhat unclear. Recently, we were unable to demonstrate a cytotoxic effect of ALS sera on human central nervous system (CNS) cultures from 15 - 20 week old human foetuses, confirming the negative therapeutic effects obtained using plasma-exchange in affected patients[l]. We decided to study the effects of ALS-CSF on human foetal motor cortex in vitro. This tissue can grow in vitro in the presence of a standard tissue culture medium which contains 10% normal CSF and provides a sensitive assay for trophic factors and toxic substances of clinical relevance. ALS-CSF induces a significant glial cell proliferation in vitro. Indirect immunofluorescence techniques were used to identify the atroglial proliferating cells.
KeywordsAmyotrophic Lateral Sclerosis Motor Cortex Amyotrophic Lateral Sclerosis Patient Motor Neuron Disease Sporadic Amyotrophic Lateral Sclerosis
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