Human Spinal Cord Neurons in Culture as a Tool to Study Amyotrophic Lateral Sclerosis

  • A. C. Kato
  • L. Erkman
  • G. Touzeau
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 209)


It has been reported that sera from amyotrophic lateral sclerosis (ALS) patients have a neuron-specific toxic effect on long-term cultures of neonatal mouse anterior horn cultures[1-3]. In these experiments, morphological rather than biochemical criteria were used as an index of cytotoxicity. Since these results could not be repeated by other groups using the same assay system or another type of in vitro preparation[4-9], we decided to examine the effects of control and disease serum on human spinal cord neurons in culture. This preparation was recently developed and characterized in our laboratory[10]. Here we describe some of the properties of these cells and the effects of serum from ALS patients on their biochemical properties.


Spinal Cord Amyotrophic Lateral Sclerosis Amyotrophic Lateral Sclerosis Patient Cholinergic Neuron Percoll Gradient 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • A. C. Kato
    • 1
  • L. Erkman
    • 1
  • G. Touzeau
    • 1
  1. 1.Department of PharmacologyCentre Médical UniversitaireGenevaSwitzerland

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