Human Spinal Cord Neurons in Culture as a Tool to Study Amyotrophic Lateral Sclerosis
It has been reported that sera from amyotrophic lateral sclerosis (ALS) patients have a neuron-specific toxic effect on long-term cultures of neonatal mouse anterior horn cultures[1-3]. In these experiments, morphological rather than biochemical criteria were used as an index of cytotoxicity. Since these results could not be repeated by other groups using the same assay system or another type of in vitro preparation[4-9], we decided to examine the effects of control and disease serum on human spinal cord neurons in culture. This preparation was recently developed and characterized in our laboratory. Here we describe some of the properties of these cells and the effects of serum from ALS patients on their biochemical properties.
KeywordsSpinal Cord Amyotrophic Lateral Sclerosis Amyotrophic Lateral Sclerosis Patient Cholinergic Neuron Percoll Gradient
Unable to display preview. Download preview PDF.
- 2.F. Wolfgram, Blind studies on the effect of amyotrophic lateral sclerosis sera on motor neurons in vitro, in: “ALS Recent Research Trends”, J. M. Andrews, R. T. Johnson, and M. A. B. Brazier, eds., pp. 145–149, Academic Press, New York, (1976).Google Scholar
- 8.M. S. Ecob, A. E. Brown, C. Young, T. Walls, D. Serisier, and P. Hudgson, Is there a circulating neurotoxic factor in motor neurone disease?, in: “Research Progress in Motor Neurone Disease”, C. F. Rose, ed., pp.249–254, Pitman Press (1984).Google Scholar
- 13.J. Malinsky and J. Malinska, Developmental stages of the prenatal spinal cord in man, Fol.Morphol.Czech.Acad., 18: 228–235 (1970).Google Scholar
- 14.G. Touzeau and A. C. Kato, ALS sera have no effect on three enzymatic activities in cultured human spinal cord neurons, Neurol., 36: 573–576 (1985).Google Scholar