Relationships of Changes in Postheparin Hepatic and Lipoprotein Lipase Activity to HDL-Cholesterol Changes Following Weight Loss Achieved by Dieting versus Exercise
Postheparin hepatic (HLA) and lipoprotein lipase activities (LPLA) were studied in moderately overweight (27.7 ± 0.47% body fat, mean ± S.E.) sedentary men aged 30–59 before and after randomization to control status (C.N = 41) or to a 1-year weight loss program by moderate dieting without exercise (D, N = 38) or exercise (running) without dieting (E, N = 44). After 6 weeks of weight stabilization at 1 year, both D and E had lost significant (P > 0.01) weight (-8.0 kg and -4.4 kg, respectively) relative to C. primarily as fat mass ( -6.0 kg and -3.8 kg), whereas only D lost nonfat mass (-2.0 kg). Compared to E, D lost more fat mass (P < 0.05) and nonfat mass (P < 0.01), but changes in percentage body fat did not differ between D and E, although these were significant relative to C ( -4.4% and -3.1%; P < 0.001). Both ΔHDL-cholesterol (HDL-C) and ΔHDL2-C were elevated (p < 0.01) in D and E versus C. but ΔHDL2-C correlated with loss of body fat in E only. Relative to C., HLA was reduced from base line (7.7 ± 0.3 mU/ml per min) in both D (-0.97 ± 0.31, P < 0.01) and E (-0.70 ± 0.30. P < 0.05). whereas increases in LPLA were not significant for D(P < 0.21) or E (P= 0.13) Both ΔHLA and ΔLPLA correlated (Spearman’s ρ) with changes in body composition and HDL-cholesterol and HDL-mass. In summary, at 1 year ΔHLA was closely associated with weight changes in D and E, whereas ΔLPLA correlated with these changes in E only. Furthermore, significant relationships for ΔHLA and ΔLPLA PLA versus ΔHDL-C and ΔHDL-mass were found only in E.
KeywordsLipase Activity Lipoprotein Lipase Hepatic Lipase Lipoprotein Lipase Activity Lipid Research Clinic
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- Applebaum-Bowden, D., Haffner, S. M., Wahl, P. W., Hoover, J. J., Warnick, G. R., Albers, J. J., and Hazzard, W. R., 1985, Postheparin plasma triglyceride lipases: Relationships with very low density lipoprotein triglyceride and high density lipoprotein cholesterol, Arteriosclerosis 5:273–282.PubMedCrossRefGoogle Scholar
- Kuusi, T., Nikkila, E. A., Saarinen, P., Varjo, P., and Laitinen, L. A., 1982, Plasma high density lipoproteins HDL2, HDL3 and postheparin plasma lipases in relation to parameters of physical fitness, Atherosclerosis 41:209–219.Google Scholar
- Lindgren, F. T., Jensen, L. C., and Hatch, F. T., 1972, The isolation and quantitative analyses of serum lipoproteins, in: Blood Lipids and Lipoproteins: Quantitation, Composition and Metabolism (G. J. Nelson, ed.), Wiley-Interscience, New York, pp. 181–274.Google Scholar
- Lipid Research Clinic Manual of Operations ,Vol. 1. Lipid and Lipoprotein Analysis ,HEW Publication No. NIH 75–628, US Government Printing Office, Washington, 1974.Google Scholar
- Nikkila, E. A., Kuusi, T., and Taskinen, M.-R., 1982, Role of lipoprotein lipase and hepatic endothelial lipase in the metabolism of high density lipoproteins: A novel concept on cholesterol transport in HDL cycle, in: Metabolic Risk Factors in Ischemic Cardiovascular Disease (L. A. Carlson and B. Pernow, eds.), Raven Press, New York, pp. 205–215.Google Scholar
- Stefanick, M. L., Frey-Hewitt, B., Hoover, C. A., Terry, R. B., and Wood, P. D., 1986, The effect of active weight loss achieved by dieting versus exercise on post-heparin hepatic and lipoprotein lipase activity, Obesity ,754:650–653.Google Scholar
- Wood, P. D., Haskell, W. L., and Fortmann, S. P., 1986, Effects on lipoproteins of weight loss by dieting versus exercise in a controlled trial,CVD Epidemiol. Newslett.39:50.Google Scholar