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Atherosclerosis and Viral Gene in Japanese Quail

  • Jason C. H. Shih
  • Roman Pyrzak
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Two lines of Japanese quail were genetically selected. One is highly susceptible (SUS) to atherosclerosis, developing severe aortic atherosclerosis in 9 weeks by feeding 0.5% cholesterol in the diet. The other is highly resistant (RES), developing little disease under the same conditions. The pathology of quail atherosclerosis, characterized by intimal thickening, the presence of foam cells, the proliferation of myofibroblast cells, and the formation of scar with collagen deposition, is similar to the human disease. Because of its small size, low feed consumption, and short life cycle, these quail are an excellent model for the study for prevention and pathogenesis of atherosclerosis. A study has shown that the quail respond to treatment with nicotinic acid, gemfibrozil, clofibrate, and aspirin with reduction of atherosclerosis. More recently these birds were tested for the presence of a herpes virus, Marek disease virus (MDV), as a possible etiologic agent. Initial diagnosis by isolation of MDV and agar precipitin test was negative. However, when a gene library of MDV was used to prepare a battery of radioactive DNA probes, high-stringency DNA hybridization has demonstrated the presence of viral genes in quail aorta, heart, nucleated RBC, and embryo. The intensity of hybridization increased with the increased severity of atherosclerosis in aortas. In embryos, all SUS quail were positive, but only a part of RES quail were positive. These results indicated that these quail were latently infected by a putative herpes virus closely related to MDV. Since DNA homology was detected in the embryo, it is possible that the viral genes are integrated in the host DNA, and they are the heritable elements for the susceptibility to atherosclerosis.

Keywords

Nicotinic Acid Herpes Virus Lipoic Acid Japanese Quail Genetic Selection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Benditt, E. P., Barrett, T., and Mc-Dougall, J. K., 1983, Viruses in the etiology of atherosclerosis, Proc. Natl. Acad. Sci. U.S.A. 80:6386–6389.PubMedCrossRefGoogle Scholar
  2. Chapman, K. P., Stafford, W. W., and Day, C. E., 1976, Animal model for experimental atherosclerosis produced by selective breeding of Japanese quail, in: Atherosclerosis Drug Discovery (C. E. Day, ed.), Plenum Press, New York, pp. 347–356.Google Scholar
  3. Day, C. E., Stafford, W. W., and Schurr, P. E., 1977, Utility of a selected line (SEA) of Japanese quail (Coturnix coturnix japonica) for the discovery of new antiatherosclerosis drugs, Lab. Anim. Sci. 27:817–821.PubMedGoogle Scholar
  4. Fabricant, C. G., 1985, Atherosclerosis: The consequence of infection with a herpesvirus, Adv. Vet. Sci. Comp. Med. 30:39–66.PubMedGoogle Scholar
  5. Gibbs, P. C., Nazerian, K., Velicer, L. F., and Kung, H. J., 1984, Extensive homology exists between Marek disease herpesvirus and its vaccine virus, herpesvirus of turkeys, Proc. Natl. Acad. Sci. U.S.A. 81:3365–3369.PubMedCrossRefGoogle Scholar
  6. Maniatis, T., Fritsch, E. F., and Sambrook, J., 1982, Molecular Cloning, A Laboratory Manual ,Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.Google Scholar
  7. McCormick, R. B., Radcliffe, J. D., Mehta, R. G., Thompson, C. A., and Moon, R. C., 1982, Temporal association between arterial cholesterol deposition, thymidine incorporation into DNA, and atherosclerosis in Japanese quail fed an atherogenic diet, Atherosclerosis 42:1–13.PubMedCrossRefGoogle Scholar
  8. Melnick, J. L., Petrie, B. L., Dreesman, G. R., Burek, J., McCollum, C. H., and De Bakey, M. E., 1983, Cytomegalovirus antigen within human arterial smooth muscle cells, Lancet 8351:644–647.CrossRefGoogle Scholar
  9. Minick, C. R., Fabricant, C. G., Fabricant, J., and Litrenta, M. M., 1979, Atherosclerosis induced by infection with a herpesvirus, Am. J. Pathol. 96:673–706.PubMedGoogle Scholar
  10. Pyrzak, R., and Shih, J. C. H., 1987, Detection of the DNA of Marek’s disease herpesvirus in Japanese quail susceptible to atherosclerosis, Atherosclerosis ,(in press).Google Scholar
  11. St’Clair, R. W., 1983, Metabolic changes in the arterial wall associated with atherosclerosis in the pigeon. Fed. Proc. 42:2480–2485Google Scholar
  12. Saunders, R. N., 1982, Evaluation of platelet-inhibiting drugs in models of atherosclerosis, Annu. Rev. Pharmacol. Toxicol. 22:276–295.CrossRefGoogle Scholar
  13. Sharma, J. M., 1980, Marek’s disease, in: Isolation and Identification of Avian Pathogens ,second ed. (S. B. Hitchner, C. H. Domermuth, H. G. Purchase, and J. E. Williams, eds.), American Association of Avian Pathologists, Department of Veterinary Microbiology, Texas A & M University, College Station, TX, pp. 91–95.Google Scholar
  14. Shih, J. C. H., 1983, Atherosclerosis in Japanese quail and the effect of lipoic acid, Fed. Proc. 42:2494–2497.PubMedGoogle Scholar
  15. Shih, J. C. H., Pullman, E. P., and Kao, K. J., 1983, Genetic selection, general characterization, and histology of atherosclerosis susceptible and resistant Japanese quail, Atherosclerosis 49:41–53.PubMedCrossRefGoogle Scholar
  16. Wagner, W. D., Clarkson, T. B., Feldner, M. A., and Prichard, R. W., 1973, The development of pigeon strains with selected atherosclerosis characteristics, Exp. Mol. Pathol. 19:304–319.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Jason C. H. Shih
    • 1
  • Roman Pyrzak
    • 1
  1. 1.Department of Poultry ScienceNorth Carolina State UniversityRaleighUSA

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